Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-125-8 | CAS number: 1303-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to fish
Administrative data
- Endpoint:
- fish short-term toxicity test on embryo and sac-fry stages
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well performed study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 981
Materials and methods
- Principles of method if other than guideline:
- no guideline was reported
- GLP compliance:
- no
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- The boron compounds used for testing were obtained from the Fisher Scientific Company. Boric acid (H3BO3) was certified ACS grade.
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Gravid fish and amphibians were obtained from State and Federal hatcheries at Frankfort, Kentucky, Erwin, Tennessee
- Boron treatment was initiated subsequent to fertilization
METHOD FOR PREPARATION AND COLLECTION OF FERTILIZED EGGS
- Method of collection of fertilised eggs: Trout eggs and sperm were collected for test purposes by artificial spawning and milking, using methods of Leitritz. Fertilization was accomplished by mixing sperm and eggs for 15 minutes immediately prior to the onset of boron exposure.
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- 197 mg/L
- Test temperature:
- 13.2 °C
- pH:
- 7.4
- Dissolved oxygen:
- 9.8 mg/L
- Nominal and measured concentrations:
- Nominal concentrations : 0.01, 0.10, 1.00, 10.00, 25.0, 50.0, 100.00, 200.00, 300.00, 400.0, 500.0 mg B/L
Measured concentrations: 0.008, 0.1, 1.26, 10.19, 24.98, 49.36, 100.0, 196.1, 383.3, 482.2 mg B/L - Details on test conditions:
- TEST SYSTEM
Fish eggs were cultured through 4 days posthatching in Pyrex chambers, through which test water was perfused at prescribed flow rates. The toxicant was administered to a mixing chamber situated ahead of each culture dish, using graduated flow from a syringe pump. Synthetic culture water was delivered to the mixing chamber by regulated flow from a peristaltic pump. Flow rates from both syringe and peristaltic pumps were monitored by liquid flow meters. Flow rate was set at 200 ml /hr for 300 ml test chambers. Syringe and peristaltic pumps were provided with calibrated, continuously variable speed controls. The concentration of toxicant delivered to the mixing chamber was regulated by adjusting the mixing ratio from the pumping units and/or by varying the concentration of toxicant delivered from the syringe pump. Solutions from the two channels were mixed with a Teflon-coated magnetic stirring bar, and delivered to the culture chamber under positive pressure.
The system was operated using Brinkmann (model 131900) and Gilson (model HP8) multichannel peristaltic pumps and Sage syringe pumps (model 355)). The latter was fitted with a modified syringe holder, and operated using 10 ml double-ground, glass syringes. Syringes were selected for equal stroke volume, and peristaltic pump channels were fitted with tubing of matched diameters.
For test concentrations of 10 ppm or more, boron compounds generally were added directly to the culture water in the peristaltic pump reservoir, eliminating the need for the syringe pump channel. In such instances, the toxicants remained stable at selected test concentrations for 24 hours or more in polyethylene containers, and important test parameters of culture water were not altered upon standing.
All aquatic bioassay cultures were maintained in walk-in environmental rooms. Culture water was given continuous aeration in the peristaltic pump reservoirs.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Culture water was prepared from distilled, double deionized water, having a conductivity of 0.25 umhos or less. Routine monitoring was conducted for background contaminants, including mercury, cadmium, lead and other trace metals. Reagent grade calcium, magnesium, sodium and potassium salts were added. The basic stock was prepared to give a water hardness level of 200 ppm (as CaCO3) and a pH of 7.5 - 8.0. Different levels of hardness were achieved by dilution of the basic culture water.
- Alkalinity: 65 mg/L
- Conductivity: 282 umhos
- Intervals of water quality measurement: daily intervals
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- LC10
- Effect conc.:
- 5.1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks:
- + teratogenicity
- Remarks on result:
- other: confidence limits: 2.4 - 10.7 mg/L
- Duration:
- 28 d
- Dose descriptor:
- LC10
- Effect conc.:
- 31.4 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- mortality
- Remarks on result:
- other: confidence limits: 10.0 - 98.5 mg/L
- Reported statistics and error estimates:
- Log probit analysis was used to statistically determine LC01 and LC50 values. The LC10/EC10 values have been recalculated with the log-logistic model using Toxicity Relationship Analysis Program, Version 1.21A
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

Route: .live1