methyl acrylate; methyl propenoate

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:

biodegradation in water: simulation testing on ultimate degradation in surface water

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is readily biodegradable

Cross-reference

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 Feb 2005 - 24 Mar 2005 (experimental)

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study conducted in compliance with GLP regulations

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)

Deviations:

no

Principles of method if other than guideline:

ISO 14593 Water quality- Evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic compounds - Method by analysis of released inorganic carbon in sealed vessels (1999).

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): from laboratory waste water treatment plant , fed with municipal sewage.
- Pretreatment: 24 h aeration
- Concentration of sludge: 4 mg/l dry substance

Duration of test (contact time):

28 d

Initial conc.:

20 mg/L

Based on:

DOC

Initial conc.:

35 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

inorg. C analysis

Remarks:

(TIC/ThIC)

Details on study design:

TEST CONDITIONS
- Composition of medium: 90 ml/vessel mineral medium, 0.5 ml/vessel inoculum and 3.7 µl/vessel test substance filled up to a total volume of 100 ml
- Test temperature: 22 +/- 2 °C
- pH: 7.,4 +/-0.2
- pH adjusted: yes (1. mol sulfuric acid)


TEST SYSTEM
- Number of culture flasks/concentration: 1
- Test performed in closed vessels due to significant volatility of test substance: yes


SAMPLING
- Sampling frequency: on days 3, 7, 10, 14, 17, 21 and 28


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes

Reference substance:

aniline

Parameter:

% degradation (inorg. C analysis)

Value:

90 - 100

Sampling time:

28 d

Remarks on result:

other: TIC/ThIC

Details on results:

Duration of adaptation phase (days): 1
Duration of degradation phase (days): 20
Degradation degree of the test substance at the end of exposure (% TIC/ThIC): 90-100
Degradation of the test substance at the end of the 10-day window (% TIC/ThIC): 80-90
Degradation degree in the inhibition control 14 days (% TIC/ThIC) 80-90
Physical-chemical (abiotic) elimination of the test substance at the end of exposure (% TIC/ThIC): <10


The test substance is readily biodegradable (according to OECD criteria).

Kinetic of test substance (in %):
= 54 after 3 day(s)
= 85 after 7 day(s)
= 86 after 10 day(s)
= 91 after 14 day(s)
= 97 after 21 day(s)
Kinetic of control substance (in %):
= 63 after 3 day(s)
= 99 after 14 day(s)

Results with reference substance:

Degradation degree of the reference substance after 14 days (% TIC/ThIC): 90-100

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable