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Ecotoxicological information

Toxicity to soil microorganisms

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Description of key information

The effect of 2-phenoxyethanol on nitrate formation resulted in a long-term EC10 of 951 mg/kg soil after 28 days, with a corresponding EC50 of >1000 mg/kg soil, the effect on carbon dioxide evolution resulted in a long-term EC10 (and EC50) of >1000 mg/kg soil and a short-term EC10(7d) of 147 mg/kg soil (with confidence limits >95%, ranging from 41 to 529). 

Key value for chemical safety assessment

Short-term EC50 for soil microorganisms:
2 636 mg/kg soil dw
Long-term EC10 or NOEC for soil microorganisms:
2 507 mg/kg soil dw

Additional information

A test on long-term potential effects of 2-phenoxyethanol on nitrate transformation was conducted according to OECD Guideline 216 under GLP (BASF SE, 2008c). Soil samples mixed with lucerne meal as source of nitrogen and placed in test vessels were exposed to 2-phenoxyethanol (purity 99.9%) at the following concentrations 0, 62.5, 125, 250, 500 and 1000 mg/kg DM in the dark for 28 days. The results were a long-term EC10 of 951 mg/kg soil after 28 days, with a corresponding EC50 of >1000 mg/kg soil. T

The percentage of organic carbon in the natural soil should be corrected for. As the percent organic carbon was 1.29% and the agreed standard organic matter percent for tests is 3.4% a correction factor of 2.636 should be multiplied by all endpoints resulting in following:

EC10 (standard): >2636 mg/kg dw soil after 28 days

EC50 (standard): >2636 mg/kg dw soil after 28 days

Another test on short- and long-term potential effects of single exposure to 2-phenoxyethanol on carbon transformation activity of soil microorganisms was conducted according to OECD Guideline 217 under GLP (BASF SE, 2008d). Soil samples were exposed to the test substance at the following (nominal) concentrations: 0, 62.5, 125, 250, 500 and 1000 mg/kg DM. Glucose-induced respiration rates were measured for 12 hours by measuring negative pressure (Oxi-Top) at 20°C in darkness. The effect on carbon dioxide evolution resulted in a long-term EC10 (and EC50) of >1000 mg/kg soil and a short-term EC10(7d) of 147 mg/kg soil (with confidence limits >95%, ranging from 41 to 529).