Registration Dossier
Registration Dossier
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EC number: 424-450-6 | CAS number: 152628-03-0
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Adequacy of study:
- other information
Data source
Reference
- Reference Type:
- other: Body responsible for the test
- Title:
- Unnamed
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: 92/69/EWG, B.14 (Ames-Test); OECD 471
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Method
Species / strain
- Species / strain / cell type:
- bacteria, other: sat TA 98, 100, 1535, 1537
- Metabolic activation system:
- Aroclor 1254 induzierter Rattenleber S9-Mix
- Test concentrations with justification for top dose:
- Concentration range in the main test (with metabolic activation): 100 ... 5000 µg/plate
Concentration range in the main test (without metabolic activation): 100 ... 5000 µg/plate - Vehicle / solvent:
- Solvent: Dimethylsulfoxid (DMSO)
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: as specified above
- Metabolic activation:
- with
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (> 5000 µg/plate)
- Species / strain:
- other: as specified above
- Metabolic activation:
- without
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (> 5000 µg/plate)
- Additional information on results:
- Observations:
Ohne metabolische Aktivierung zeigten alle 4 Stämme über den
gesamten Prüfbereich keinen dosisabhängigen Anstieg der Zahl
der Revertant-Zellen. Gleiches gilt auch für TA 1535 mit
metabolischer Aktivierung.
Mit metabolischer Aktivierung zeigen die Stämme TA 1537,
TA 98 und TA 100 einen deutlichen dosisbezogenen Anstieg der
Zahl der Revertant-Kolonien.
"ENGLISH"
Without metabolic activation, none of the four strains
exhibited any dose-dependent increase in the number of
revertant cells throughout the entire test period. The same
also applies to TA 1535 with metabolic activation.
With metabolic activation, the strains TA 1537, TA 98 and TA
100 revealed a clear dose-dependent increase in the number
of revertant colonies. - Remarks on result:
- other: other: preliminary test
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
negative without metabolic activation
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