Triethylamine

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02. Oct 1956 - 19. Oct 1956

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented report which meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1960

Report date:

1960

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

50 µL was used

Principles of method if other than guideline:

Method: BASF-Test. 50 µL of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after 10 min, 1 and 3h on the day of treatment and up to 19 days afterwards. Findings were recorded daily. The eyes were not washed out after 24 hours as specified in OECD Guideline 405. The report describes findings after 1 and 24 hours and after 8 days. For final evaluation, the findings after 48 and 72 hours from the raw data were taken into account.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Triethylamine

EC Number:

204-469-4

EC Name:

Triethylamine

Cas Number:

121-44-8

Molecular formula:

C6H15N

IUPAC Name:

triethylamine

Details on test material:

- Name of test material (as cited in study report): Triaethylamine

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 1.94 and 2.38 kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: single application, eyes were not washed out

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1 drop (ca. 50 µl)

Duration of treatment / exposure:

single application, eyes were not washed out

Observation period (in vivo):

19 days

Number of animals or in vitro replicates:

2

Results and discussion

In vivo

Results

Irritation parameter:

other:

Basis:

mean

Time point:

other: 24 h - 48 h - 72 h

Reversibility:

not reversible

Remarks on result:

other: test substance caused corneal opacity

Any other information on results incl. tables

The application of the test substance caused after 10 minutes severe corneal opacity, bleeding of the nictating membrane, conjunctival edema, chemosis and redness. The symptoms were persisting for the next 2 weeks. The corneal opacity is not regarded to be reversible.

Applicant's summary and conclusion

Interpretation of results:

highly irritating

Remarks:

Migrated information or corrosive Criteria used for interpretation of results: expert judgment

Conclusions:

triethylamine caused severe ocular injury.

Executive summary:

The BASF study was similar to the OECD Guideline 405. The duration of the study was suffucient to evaluate reversibility of the effects. Triethylamine was applied to the conjunctival sac of one rabbit eye to assess eye irritation potential. the test substance caused irreversible eye opacity. The effects occured after 10 min of treatment.