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EC number: 204-694-8
CAS number: 124-28-7
Identification of Strain MA3
An isolate was obtained in pure culture by streaking the enrichment
culture on agar plates containing mineral salts and dodecyldimethylamine.
Oxidative cleavage of C alkyl-N bond
Cells grown on dodecyldimethylamine readily oxidized
dodecyldimethylamine to dimethylamine, dodecanal and dodecanoic acid.
Pseudomonas MA3 is capable to use dodecyldimethyl amine as sole carbon
and energy source oxidizing and cleaving the substance to dodecanal,
dodecanoic acid and dimethylamine. Dodecanoic acid as a fatty acid is
subsequently biodegraded by beta oxidation. The C alkyl - N bond is
cleaved with help of a dehydrogenase. The metabolites dodecanal and
dodecanoic acid were identified using specific enzyme inhibitors to stop
further metabolism. Dimethylamine was determined by GC.
Finally the intermediate metabolites will be mineralised which means
that Dodecyldimethylamine is ultimately biodegraded.
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