Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-002-4 | CAS number: 112-75-4
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
In a reliable study (reliability category 1) assessing long-term effects of the supporting substance HYEQS on nitrogen transformation rate of terrestrial microorganisms, after 28 days no inhibitory effects were determined (NOEC ≥ 1000 mg/kg soil dry weight).
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 1 000 mg/kg soil dw
Additional information
Long-term effects on nitrogen transformation rate of terrestrial microorganisms after single exposure to the supporting substance HYEQS was assessed in a reliable (category 1) study performed according to OECD 216 and compliant to GLP over a period of 28 days. The validity criterion of the guideline was met.
HYEQS is of high similarity to C12-14 DMA in that it has essentially the same alkyl chain distribution and – like DMA category members - two methyl substituents at the amino group. The only difference is an additional hydroxyethyl substituent at the amino group rendering it a quaternary amine being permanently positively charged. This is however a minor difference as due to protonation at environmentally relevant pH values also DMA category members are predominantly positively charged at the amino group.
HYEQS was tested at five different application rates between 25.6 and 1000 mg/kg soil dry weight. The NOEC, EC50 and EC25 for nitrogen transformation rate in soil measured as nitrate on day 7 was 400, > 1000 and 782 mg/kg soil dry weight, respectively.
The NOEC, EC50 and EC25 for nitrogen transformation rate in soil measured as nitrate on day 14 was 400, 908 and 562 mg/kg soil dry weight, respectively.
The NOEC for nitrogen transformation rate in soil measured as nitrate on day 28 was≥1000 mg/kg soil dry weight.
In conclusion,no long-term inhibitory effects of DMA category members on the nitrogen transformation rate of terrestrial microorganisms are expected, even at high exposure concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
