3-Quinolinecarbonitrile, 4-[(2,4-dichloro-5-methoxyphenyl)amino]-6-methoxy-7-[3-(4-methyl-1-piperazinyl)propoxy]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10-12 August 2009

Reliability:

1 (reliable without restriction)

Data source

Reference

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: ISO International Standard 6341: "Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus - Acute toxicity test, Third edition, 1996-04-01.

Deviations:

no

Test guideline 4

Qualifier:

according to guideline

Guideline:

other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-[(2,4-dichloro-5-methoxyphenyl)amino]-6-methoxy-7-[3-(4-methylpiperazin-1-yl)propoxy]quinoline-3-carbonitrile

EC Number:

700-455-1

Cas Number:

380843-75-4

Molecular formula:

C26H29Cl2N5O3

IUPAC Name:

4-[(2,4-dichloro-5-methoxyphenyl)amino]-6-methoxy-7-[3-(4-methylpiperazin-1-yl)propoxy]quinoline-3-carbonitrile

Sampling and analysis

Details on sampling:

During the combined limit/range-finding test singular samples for possible analysis were taken from all test concentrations and the control according to the schedule below. In addition, the filter containing the undissolved residue was stored for possible analysis. The method of analysis is described in the appended Analytical Report (Appendix II).
Frequency at t=0 h and t=48 h
Volume 9 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until analysis.
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Additionally, reserve samples of 9 ml were taken for possible analysis. If not used, these samples were stored in a freezer for a maximum of three months after delivery of the draft report, pending the decision of the sponsor for additional analysis.

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

not specified

Total exposure duration:

48 h

Test conditions

Test temperature:

Temperature of medium 18-22°C

Details on test conditions:

Test procedure and conditions
Test duration 48 hours
Test type Static
Test vessels 100 ml, all-glass
Medium Adjusted ISO medium
Number of daphnids Control and highest test group: 20 per concentration Lower test groups; 10 per concentration
Loading 5 per vessel containing 80 ml of test solution
Light 16 hours photoperiod daily
Feeding No feeding
Aeration No aeration of the test solutions.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.43 mg/L

Basis for effect:

mobility

Applicant's summary and conclusion

Conclusions:

Under the conditions of the present study WAY-173606 (crystallized) did not induce acute immobilisation of Daphnia magna at 0.48 mg/l after 48 hours of exposure (NOEC).
The 48h-EC50 was beyond the range tested, i.e. exceeded an initially measured concentration of 0.48 mg/l.
Due to the very low solubility of WAY-173606 (crystallized) in water, concentration levels that might be toxic for Daphnia magna could not be reached.