Registration Dossier
Registration Dossier
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EC number: 204-445-3 | CAS number: 121-03-9
Endpoint summary
Administrative data
Description of key information
irritating to skin
causes serious damage to eyes
Key value for chemical safety assessment
Skin irritation / corrosion
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Eye irritation
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Additional information
Skin irritation /corrosion
4-nitrotoluene-2-sulphonic acid was irritating to the skin in the Epiderm TM Skin Corrosion/Irritation Test (OECD 431) (BASF SE, 2010). For the corrosion test the samples were incubated with the test substance for 3 minutes and 1 hour, for the irritation test the samples were incubated with the test substance for 1 hour followed by a 42 -hours post-incubation period. Tissue destruction was determined by measuring the metabolic acitvity of the tissue after exposure/post-incubation using a colorimetric test. The reduction of mitochondrial dehydrogenase activity, measured by reducted formazan production after incubation with a tetrazolium salt (MTT) was chosen as suitable endpoint.
The mean viability of the test substance treated tissues determined after an exposure period of 3 minutes was 101%, and it was 17% after an exposure period of 1 hour.The mean viability of the test-substnce treated tissues determined after an exposure period of 1 hour with about 42 hours post-incubation was 7%.
Eye irritation
4-nitrotoluene-2-sulphonic acid causes serious eye damage in the Bovine corneal Opacity and Permeabilit Tet (OECD 437) (BASF SE, 2010). Corneal opacity was measured quantitatively as the amount of light transmission through the cornea. Permeability was measured quantitatively as the amount of sodium fluorescein dye that passes across the full thickness of the cornea. The mean Opacity change of the test-substance treted corneas was 143.4. The mean OD490 of the test-substance treateed corneas determined as permeability parameter was 0.013. The In Vitro Irritancy Score (IVIS) of the test-substance treated corneas was calculated to be 143.6.
Justification for classification or non-classification
The evaluation of classification for skin and eye irritation is based on the available in vitro tests.
As per the OECD 431, the tissue viability suggests that the substance is a skin irritant.
As per the OECD 437, the IVIS score being higher than 50, suggests that the substance causes serious damage to eyes.
According to the CLP Regulation EC No. 1272/2008, the substance is classified as:
- serious eye damage, category 1 (H318)
- skin irritation, Category 2 (H315)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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