Isopropylamine

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1989

Report date:

1989

Reference 2

Reference Type:

publication

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: American Society for Testing and Materials (ASTM), Standard Test Method for Estimating Sensory Irritancy of Airborne Chemicals, Annual Book of ASTM Standards, Designation E 981-84

Principles of method if other than guideline:

Determination of sensory irritation potency by oronasal exposure, key parameter: RD50
(RD50 = the exposure concentration resulting in a 50-% decrease in the respiratory rate).

GLP compliance:

not specified

Type of method:

in vivo

Endpoint addressed:

respiratory irritation

Test material

Test material information

Constituent 1

Reference substance name:

Isopropylamine

EC Number:

200-860-9

EC Name:

Isopropylamine

Cas Number:

75-31-0

Molecular formula:

C3H9N

IUPAC Name:

propan-2-amine

Test material form:

liquid

Details on test material:

high-purity liquid

Test animals

Species:

mouse

Strain:

other: OF1

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: IFFA Credo, Domaine des Onciens, Saint-Germain sur l'Arbresle, France
- Age at study initiation:
- Weight at study initiation: 20 - 25 g
- Fasting period before study: unfasted
- Housing: in groups of 10
- Diet., water: ad libitum
- Acclimation period: 7 d

controlled ENVIRONMENTAL CONDITIONS (no detailed information given)

Administration / exposure

Route of administration:

inhalation

Vehicle:

unchanged (no vehicle)

Details on exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION (see details below)
- Exposure apparatus: 200 L inhalation chamber
- Method of holding animals in test chamber: Mice were restrained in a body plethysmographs with nose-only exposure.
- Source and rate of air:
- Method of conditioning air:
- System of generating particulates/aerosols:
- Temperature, humidity, pressure in air chamber:
- Air flow rate:
- Air change rate:
- Method of particle size determination:
- Treatment of exhaust air:


TEST ATMOSPHERE
- Brief description of analytical method used: HPLC ( sampling by silica gel in glass tubes; after derivatisation resulting m-Toluene derivatives were
analysed by reversed-phase chromatography with UV detection)
- Samples taken from breathing zone: yes

Analytical verification of doses or concentrations:

yes

Duration of treatment / exposure:

Exposure period: 60 min

Frequency of treatment:

1x, 15 min

Post exposure period:

Recovery period: 30 min

Doses / concentrations

Remarks:

Doses / Concentrations:
Several concentrations measured from 69 - 197 ppm
Basis:
analytical conc.

No. of animals per sex per dose:

6 per test concentration

Control animals:

other: internal control = each test animal before exposure

Details on study design:

Observation period: 60 min exposure, 30 min recovery, before, during and after exposure respiratory rate was determined

Results and discussion

Any other information on results incl. tables

The RD50 value for isopropylamine was 157 ml/m3 (ppm) which corresponds  
to approx. 0.39 mg/L. 

Note: Calculation based on MW = 59 and molar volume of 24 L (20 °C): 
1 ml/m3 = 2.46 mg/m3
 ---------------------

Applicant's summary and conclusion

Conclusions:

Under the conditions of this study, the test substance produced sensory upper airway irritation, like related amines