2-bromopropane

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Cited experiment was summarized in the National Toxicology Program-Center for the Evaluation of Risks to Human Reproduction Monograph on the Potential Human Reproductive and Developmental Effects of 2-Bromopropane; original study was not reviewed

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

review article or handbook

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-bromopropane

EC Number:

200-855-1

EC Name:

2-bromopropane

Cas Number:

75-26-3

Molecular formula:

C3H7Br

IUPAC Name:

2-bromopropane

Method

Target gene:

Histidine operon

Test concentrations with justification for top dose:

Preliminary (50-5,000 ug/ plate) and secondary (313-5,000 ug/plate)

Results and discussion

Any other information on results incl. tables

A dose-related increase in mutations was noted with 2-bromopropane treatment in strain TA100 with S9 activation and in TA1535 with and without activation. Results in E. coli and the other Salmonella strains were negative. The authors stated that mutation in strains TA100 and TA1535 indicate base-pair substitutions.

There was a mutagenic effect in two strains of Salmonella, one only with metabolic activation, one with and without metabolic activation. The two strains both detect base-pair substitutions, raising the likelihood that 2-bromopropane, at least under some circumstances, is mutagenic.

Applicant's summary and conclusion

Conclusions:

In this supporting study, dose-related increases in mutations were noted with 2-bromopropane treatment in S. typhimurium strain TA100 with S9 activation and in TA1535 with and without activation. These results indicated that 2-bromopropane is mutagenic under certain conditions.