2-bromopropane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Full study report not available in English (English summary only); therefore, not all information could be verified.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-bromopropane

EC Number:

200-855-1

EC Name:

2-bromopropane

Cas Number:

75-26-3

Molecular formula:

C3H7Br

IUPAC Name:

2-bromopropane

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Oryzias latipes

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

See Table 6, in 'Attached Background Material"

pH:

See Table 8, in 'Attached Background Material"

Dissolved oxygen:

See Table 7, in 'Attached Background Material"

Nominal and measured concentrations:

See Table 1, in 'Attached Background Material"
Nominal concentrations: control, 10.0, 18.0, 32.0, 56.0, 100.0 mg/L (geometric ratio approximately 1.8)

Details on test conditions:

TEST SYSTEM
- Type: Covered the surface of the solutions by teflon seat.
- Fill volume: 5 L/vessel
- Renewal rate of test solution (frequency/flow rate): All test solutions exchanged every 24 hours
- No. of organisms per vessel: 10/concentration
- No. of vessels per concentration (replicates): 1 vessel/concentration
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water quality of dilution water presented in 'Attached Background Material", source not given.
OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark
- Test concentrations: Nominal concentrations: control, 10.0, 18.0, 32.0, 56.0, 100.0 mg/L (geometric ratio approximately 1.8)

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 66.6 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

See Table 2, 3, 4, 5, in 'Attached Background Material".
The LC50 value and its 95% confidence limits could not be determined by statistical method because the mortality of Medaka at the maximum
concetration level was less than 50%.

Any other information on results incl. tables

Sublethal observations / clinical signs:

See Table 2, 3, 4, 5, in 'Attached Background Material". The LC50 value and its 95% confidence limits could not be determined by statistical method because the mortality of Medaka at the maximum concetration level was less than 50%.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-hr LC50 was >66.6 mg/L based on geometric mean measured concentration. Results should be interpreted with caution because the test substance concentration was not maintained above 80% of the initial concentration.

Executive summary:

The 96 -hour toxicity of 2 -bromopropane to Oryzias latipes was studied under semi-static conditions in accordance with the OECD Guidelines for the Testing of Chemicals, No. 203, Fish Acute Toxicity Test. Fish were exposed to 2 -bromopropane at nominal concentrations of: control, 10.0, 18.0, 32.0, 56.0, 100.0 mg/L (geometric ratio approximately 1.8). Mortality effects were observed every 24 hours.

RESULTS SYNOPSIS*:

The 96 hour LC₅₀was > 66.6 mg/L.

*Results should be interpreted with caution because the test substance concentration was not maintained above 80% of the initial concentration. However, the LC50 is based on geometric mean measured concentration, according to guidelines.