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EC number: 404-310-0 | CAS number: 10591-85-2 PERKACIT TBZTD; PERKACIT TBZTD PDR; PERKACIT TBZTD PDR-D; TBZD
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: As all validation criteria are fulfilled the present study is valid without restrictions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- other: In addition the instructions of the OECD series on testing and assessment, no. 23: Guidance document on aquatic toxicity testing of difficult substances and mixtures" (ENV/JM/MONO(2000)6) were followed.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tetrakis(phenylmethyl)thioperoxydi(carbothioamide)
- EC Number:
- 404-310-0
- EC Name:
- Tetrakis(phenylmethyl)thioperoxydi(carbothioamide)
- Cas Number:
- 10591-85-2
- Molecular formula:
- C30H28N2S4
- IUPAC Name:
- N,N-dibenzyl[(dibenzylcarbamothioyl)disulfanyl]carbothioamide
- Details on test material:
- - Name of test material (as cited in study report): Linkwell TBzTD -Tetrakis(phenylmethyl)thioperoxydi(carbothioamid)
- Substance type: mono-constituent substance
- Physical state: solid, powder
- Analytical purity: > 99%
- Lot/batch No.: 08060902
- Expiration date of the lot/batch: 2009-06
- Storage condition of test material: room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure:
A fresh sample of activated sludge from the aeration basin of a municipal sewage treatment plant (Moers, Germany) was collected one day before carrying out the Activated Sludge Respiration Inhibition Test and aerated overnight until usage for the test.
After determination of the suspended solids (dry weight), 5.859 l activated sludge with a dry matter content of 2.048 g/l (which is equivalent to 12 g dry substance I 3 l) was filtered by means of a water jet pump through a filter paper (Schleicher & Schuell, black ribbon 589(1 )).
The sludge was washed twice with mineral medium. Afterwards, 4 g/l activated sludge (dry matter) was suspended in mineral medium, 50 mill synthetic sewage were added and the whole mixture was stirred and aerated (0.5 - 1.0 litre per minute) overnight at 20 ± 2°C. It was not necessary to buffer the pH of the inoculum suspension before use with sodium hydrogencarbonate solution, as, with a measured pH value of 7.87, it was within the required range of 6.0 to 8.0
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- inoculum suspension 7.87
others: see Table 1 - Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 l beakers (3-hour contact period) and 300 ml Karlsruher flasks (O2 measurement)
- Aeration: synthetic air, flow meter
OTHER TEST CONDITIONS
- Adjustment of pH:
EFFECT PARAMETERS MEASURED:
Oxygen consumption was followed by continous measurement of the oxygen concentration with an oxygen electrode for up to 10 minutes.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Test concentrations: 10, 32, 100, 320, 1000 mg/l (nominal) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
As the test substance is not soluble in water, each test concentration was prepared individually, stirred for 24 h and the remaining substance particles were removed by filtration. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3 h) = 14.9 mg/l is in the acepted range 5 to 30 mg/l
Any other information on results incl. tables
Table 2: Respiration rates and inhibition of respiration
|
Concentration [mg/l] |
Respiration rate |
Inhibition |
control 1 |
0 |
23.4 |
- |
control 2 |
0 |
22.8 |
- |
mean control 1 + 2 |
0 |
23.1 |
- |
3,5-dichlorophenol |
3.2 |
17.4 |
|
3,5-dichlorophenol |
10 |
16.2 |
|
3,5-dichlorophenol |
32 |
6.0 |
|
Linkwell TBzTD |
10 |
26.5 |
0 |
Linkwell TBzTD |
32 |
23.4 |
0 |
Linkwell TBzTD |
100 |
27.7 |
0 |
Linkwell TBzTD |
320 |
24.0 |
0 |
Linkwell TBzTD |
1000 |
20.9 |
9.5 |
Applicant's summary and conclusion
- Executive summary:
Linkwell TBzTD - Tetrakis(phenylmethyl)thioperoxydi(carbothioamid) was tested in an activated sludge respiration inhibition test after a 3-hour contact period according to the OECD Guideline for Testing of Chemicals, No. 209, to determine whether the test substance is toxic to the microorganisms of activated sludge.
As the test substance is not soluble in water, the test was performed with each test concentration of Linkwell TBzTD - Tetrakis(phenylmethyl)thioperoxydi(carbothioamid) prepared individually, stirred for 24 hand undissolved particles were removed by filtration.
The concentrations tested were 10, 32, 100, 320 and 1000 mg/L. Additionally two inoculum controls, one at the start and one at the end of the experiment, and 3,5-dichlorophenol in concentrations of 3.2, 10 and 32 mg/L as reference substance were tested.
The respiration rate of the activated sludge in the test vessels containing up to 1000 mg/L Linkwell TBzTD - Tetrakis(phenylmethyl)thioperoxydi(carbothioamid) (as test substance preparation) was not inhibited in comparison to the mean of the inoculum controls. Only at 1000 mg/L an inhibition rate of 9,5 % was observed, thus demonstrating that the EC50 is
above the highest tested concentration value.
EC50 > 1000 mg/L (test substance preparation)
The 3-hour EC50 for the reference substance 3,5-dichlorophenol was within the tolerable range of 5 - 30 mg/L. The deviation between the two inoculum controls was 1.8 % and therefore below the maximum permissible deviation of 15 %.
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