Hydroquinone

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Study meets basic scientific principles of an acute toxicity study reporting on mortalities and clinical signs of intoxication occurring within 24 h post application, no information on purity of the test substance, sufficient documentation of test results to be accepted as key study

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1948

Report date:

1948

Materials and methods

Principles of method if other than guideline:

Observation of mortalities and clinical signs of intoxication occuring within 24 h after i.p. application of two different grades of HQ

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hydroquinone

EC Number:

204-617-8

EC Name:

Hydroquinone

Cas Number:

123-31-9

Molecular formula:

C6H6O2

IUPAC Name:

hydroquinone

Details on test material:

- Name of test material (as cited in study report): Hydroquinone TEC pure (food grade), Hydroquinone photographic (photo grade)
- Analytical purity: no data
- Batch: no data

Test animals

Species:

rat

Strain:

other: albino

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

78 - 300 mg/kg bw as 1% solution

No. of animals per sex per dose:

5 for each grade of HQ

Details on study design:

- Duration of observation period following administration: 24 h
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

160 mg/kg bw

Based on:

test mat.

Remarks on result:

other: HQ food grade

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

175 mg/kg bw

Based on:

test mat.

Remarks on result:

other: HQ photo grade

Mortality:

HQ TEC pure: 0% up to 135 mg/kg; 40% at 135 mg/kg (2nd experiment); 40% at 154 mg/kg; 100% at 202 mg/kg; 80% at 225 mg/kg; 60% at 230 and 300 mg/kg; deaths occurred also several hrs p.a.
HQ photo grade: 0% at 81 mg/kg; 40% at 114 and 164 mg/kg; 60% at 210 mg/kg; 100% at 225 mg/kg; 80% at 229 mg/kg; 60% at 263 mg/kg; 80% at 266 and 269 mg/kg; deaths occurred witin 10 to 30 min p.a.

Clinical signs:

Clonic convulsive movements proceeding into running movements; heart observed to beat for several minutes after respiration ceased

Applicant's summary and conclusion

Conclusions:

After intraperitoneal injection LD50 values in the range of 160 or 175 mg/kg bw were found in male or female Albino rats.

Executive summary:

Mortalities and clinical signs of intoxication were observed in male and female albino rats after i.p.application of two different grades of HQ (food grade and photographic grade) for a total observation period of 24 hrs. In male and female rats, LD₅₀ values were in the range of 160 or 175 mg/kg bw for the food grade and photographic grade, respectively. These values were found to be similar to intraperitoneal LD₅₀ values of other species, as e.g. mice, rabbits, and guinea pigs. Clinical signs were characterized by clonic convulsive movements proceeding into running movements pointing to central nervous system effects.