2,2,6,6-tetramethyl-4-piperidone

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 87/302/EEC page 118-122 Activated sludge respiration inhibition test

Deviations:

yes

Remarks:

Instead of a centrifuged sludge a settled sludge was used

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2,6,6-tetramethyl-4-piperidone

EC Number:

212-554-2

EC Name:

2,2,6,6-tetramethyl-4-piperidone

Cas Number:

826-36-8

Molecular formula:

C9H17NO

IUPAC Name:

2,2,6,6-tetramethylpiperidin-4-one

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 14/09/92. The pH after collection was 7.2.
The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. The pH of the sludge before use was 7.8
The sludge concentration in the test bottles was 1.62 g/L (dry weight).

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Test conditions

Test temperature:

20 +/- 2°C

pH:

8.2

Nominal and measured concentrations:

- Concentrations of the test substance (nominal): 1, 3.2, 10, 32.1 and 100.4 mg/L
- Concentrations of the reference substance (nominal): 3.2, 10 and 32 mg/L

Details on test conditions:

- Vessels: 250 ml BOD flasks with gas inlet
- Water: Dechlorinated drinking water
- Nutrien Solution (dissolved in one liter):
16 g Peptone
11 g Meat extract
3.0 g Urea
0.7 g NaCl
0.4 g CaCl2 x 2H2O
0.2 g MgSO4 x 7H2O
2.8 g K2HPO4

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Results and discussion

Effect concentrations

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

EC50 (180 min) > 100 mg/L

Results with reference substance (positive control):

EC50 (180 min) = 15.4 mg/L