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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study according to guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Triacetonamin, TAA
- Physical state: yellow-brown paste
- Analytical purity: 69.9%
- Lot/batch No.: Pt 13800

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Strain: IRCHA
- Source: in-house breed
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: females kept in beakers filled with M4-medium according to Elendt (1990)
- Feeding during breeding:
- Food type: Scenedesmus subspicatus
- Amount: as required
- Frequency: daily
- Feeding during test: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.5 mmol (sum of Ca2+ and Mg2+)
Test temperature:
20 °C +/- 1°C
pH:
7.8-8.6
Dissolved oxygen:
8.1-8.3 mg/L
Salinity:
no data
Nominal and measured concentrations:
0, 110, 160, 220, 320, 450, 650 and 900 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, fill volume: glas vessels with 10 mL volume
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL per test organism

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater
- Chlorine: CaCl2 x H2O 294 mg/L, KCl 5.5 mg/L
- Ca/Mg ratio: 4:1

OTHER TEST CONDITIONS
- Photoperiod: dark
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
160 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
281.2 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Remarks on result:
other: 246.1 - 321.3 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
650 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Results with reference substance (positive control):
40% immobility at 0.9 mg/L, 100% immobility at 1.9 mg/L after 24 h exposure to potassium dichromate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of this daphnia immobilization study the test item triactonamine showed an EC50 of 281.2 mg/L (95% CL: 246.1-321.3 mg/L).