Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-825-9 | CAS number: 127-18-4
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: non-GLP, non-guideline study, published data, some restrictions (e.g. no details on substance purity), acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Genotoxicity of organic chemicals frequently found in the air of mobile homes
- Author:
- Connor TH, Theiss JC, Hanna HA et al
- Year:
- 1 985
- Bibliographic source:
- Toxicol Lett. 25; 33-40.
Materials and methods
- Principles of method if other than guideline:
- The mutagenicity assay employed was that described by Maron and Ames (1983) using Salmonella typhimurium strains TA100 and TA98, which are DNA-repair deficient, and two additional strains, UTH8414 and UTH8413, developed by Matney (1983) which have full DNA repair capacity.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tetrachloroethylene
- EC Number:
- 204-825-9
- EC Name:
- Tetrachloroethylene
- Cas Number:
- 127-18-4
- Molecular formula:
- C2Cl4
- IUPAC Name:
- tetrachloroethene
- Details on test material:
- - Name of test material (as cited in study report): tetrachloroethlylene- Source: Eastman, KodakNo further details on the test material were reported.
Constituent 1
Method
- Target gene:
- his
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Additional strain / cell type characteristics:
- other: DNA-repair deficient
- Species / strain / cell type:
- S. typhimurium TA 100
- Additional strain / cell type characteristics:
- other: DNA-repair deficient
- Species / strain / cell type:
- other: UTH8414
- Additional strain / cell type characteristics:
- other: DNA repair capacity
- Species / strain / cell type:
- other: UTH8413
- Additional strain / cell type characteristics:
- other: DNA repair capacity
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9, liver homogenate from Aroclor-induced male Sprague-Dawley rats and the necessary co-factors
- Test concentrations with justification for top dose:
- 50, 100, 500, 1000, and 2000 μg/plate
- Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Migrated to IUCLID6: for TA100 without S9
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: cisplatin (for UTH8414 and UTH8413 without S9)
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (for TA98 with S9)
- Details on test system and experimental conditions:
- The plate incorporation assay was performed.The concentration of S9 was 100 μl/plate. Plates not receiving S9 were prepared with a similar volume (0.5 ml) of sterile buffer.The plates were incubated at 37 ºC for 48 h, at which time the number of colonies per plate was determined. The chemical to be tested was prepared immediately prior to use and diluted in DMSO.The chemical and plates were protected from exposure to light during all procedures, including incubation.
- Evaluation criteria:
- not reported, see Maron and Ames (1983) and Matney (1983)
- Statistics:
- not specified
Results and discussion
Test results
- Species / strain:
- other: TA98, TA100, UTH8413 and UTH8414
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
