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EC number: 202-643-4
CAS number: 98-16-8
Table 1. Mean OD540 Values and Viabilities for the Negative Control
Item, Positive Control Item and Test Item
the study was performed to OECD TG 431 under GLP conditions to assess the corrosive potential of the test material to Reconstructed Human Epidermis (RHE) following single applciation. A volume of 50ul of the test item wsa applied topically to duplicate tissues ensuring uniform coverage of the tissues. duplicate tissues were also treated with 50ul of negative control 0.9% sodium chloride solution and positive control glacial acetic acid respectively. The RHE tissues samples were exposed to the test substance for 3, 60 and 240 minutes respectively with RHE tissues exposed to negative and positive controls for 240 minutes. Following the respective exposure times the tissues were rinsed for 40 seconds with PBD and incubated for 3 hours plus/minus 5 minues at room temperature. Under the conditions of this study, the test item was considered to not to be corrosive to the skin.
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