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EC number: 202-597-5
CAS number: 97-63-2
This study was performed to investigate the potential of Ethyl
methacrylate to induce gene mutations according to the plate
incorporation test (experiment I) and the pre-incubation test
(experiment II) using the Salmonella typhimurium strains
TA 1535, TA 1537, TA 98, TA 100, and the Escherichia coli strain
The assay was performed in two independent experiments both with and
without liver microsomal activation. Each concentration, including the
controls, was tested in triplicate. The test item was tested at the
Pre-Experiment/ Experiment I: 3; 10; 33; 100; 333; 1000; 2500; and 5000
Experiment II: 33; 100; 333; 1000; 2500; and 5000 µg/plate
No precipitation of the test item occured up to the highest investigated
The plates incubated with the test item showed normal background growth
up to 5000 µg/plate with and without S9 mix in all strains used.
Toxic effects, evident as a reduction in the number of revertants (below
the indication factor of 0.5), occured in experiment I in strain TA 1537
in the absence of S9 mix and in experiment II in strains TA 1535, TA
1537, and TA 98 in the presence and absence of S9 mix and in strain WP2
uvrA in the absence of S9 mix.
No substantial increase in revertant colony number of any of the five
tested strains was observed following treatment with Ethyl methacrylate
at any dose, neither in the presence nor absence of metabolic activation
(S9 mix). There was also no tendency of higher mutation rates with
increasing concentrations in the range below generally acknowledged
border of biological relevance.
Appropriate reference mutagens were used as positive controls and showed
a distinct increase of induced revertant colonies.
In conclusion, it can be stated that during the described mutagenicity
test and under the experimental conditions reported, the test item did
not induce gene mutations by base par changes or frameshifts in the
genome of the strains used.
Therefore, Ethyl methacrylate is considered to be non-mutagenic in this Salmonella
typhimurium and Escherichia coli reverse mutation assay.
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