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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study meets generally accepted scientific principles, acceptable for assessment. Vapour generation and analysis not well documented.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
yes
Remarks:
- limit test concentration: 5 mg/L
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 870.1300 (Acute inhalation toxicity)
GLP compliance:
yes
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2,4-trimethylpentane
EC Number:
208-759-1
EC Name:
2,2,4-trimethylpentane
Cas Number:
540-84-1
Molecular formula:
C8H18
IUPAC Name:
2,2,4-trimethylpentane
Details on test material:
- Name of test material (as cited in study report): Isooctane
- Analytical purity: 100% pure commercial product

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Duration of exposure:
4 h
Concentrations:
33.52 mg/L nominal concentration
21561.5 ± 776.32 ppm mean analytical concentration
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: day 2, 3, and 4 mentioned, not further specified
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Statistics:
not reported

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 33.52 mg/L air (nominal)
Exp. duration:
4 h
Mortality:
No mortality occurred during the 14-day observattion period.
Clinical signs:
other: Exposure-related observations noted in all animals during the exposure period included lying prostrate in the cage and rapid respiration. All animals appeared normal throughout the post exposure period.
Body weight:
Sightly decreased body weights were noted in the males on day 2 post exposure and in the females on days 2, 3 and 4 post exposure.
Gross pathology:
No abnormal gross pathology observations were noted in any animals upon necropsy.

Any other information on results incl. tables

Inhalation LC50 > 33.52 mg/L air (nominal) for 4 hours in male and female rats.

Applicant's summary and conclusion

Interpretation of results:
other: not classified
Remarks:
Criteria used for interpretation of results: other: GHS, EU, 2007
Conclusions:
Based on the study design the test substance, 2,2,4-trimethylpentane, needs not to be classified.
Executive summary:

Based on the study design the test substance, 2,2,4 -trimethylpentane needs not to be classified.