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EC number: 202-426-4 | CAS number: 95-51-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988-05-10 to 1988-06-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Version / remarks:
- cited as 84/449 EWG
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-chloroaniline
- EC Number:
- 202-426-4
- EC Name:
- 2-chloroaniline
- Cas Number:
- 95-51-2
- Molecular formula:
- C6H6ClN
- IUPAC Name:
- 2-chloroaniline
- Details on test material:
- - Name of test material (as cited in study report): o-Chloranilin
- Physical state: yellow-orange, clear liquid
- Analytical purity: 99.6 % (IR spectroscopy)
- Impurities (identity and concentrations):
<0.01 % cyclohexylamine
<0.01 % N-isopropylaniline
<0.01 % 2-chloro-N-Isopropylanilin
0.08% aniline
<0.01% 2-Isopropylaniline
<0.01% o-nitrochlorobenzene
0.24% p-chloroaniline
<0.01% m-chloroaniline
<0.01% 2,4-dichloroaniline
<0.01% 2,5-dichloroaniline
<0.01% 2,3-dichloroaniline
<0.01% 2,3-dichloroaniline
<0.01% o-phenylendiamine
0.03 % unknown impurities
- Purity test date: 1 Feb 1988
- Lot/batch No.: Lagerkessel 15/ 594 488
- Stability under test conditions: stable throughout the test period
- Storage condition of test material: room temperature, dark
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen
- Age at study initiation: males about 10 weeks; females: 14 weeks
- Weight at study initiation: males: about 251 g; females: about 198 g
- Fasting period before study:
- Housing: macrolon cages typ II wood chips (ssniff)
- Diet: ad libitum, Altromin 1324
- Water: ad libitum, tap water
- Acclimation period: 5 d
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2
- Humidity (%): 50 +7- 10
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: dorso-lateral
- % coverage: 10, shaved 24 h prior to application
- Type of wrap if used: gauze, tin foil and non-irritant tape
REMOVAL OF TEST SUBSTANCE
- Washing (if done): withhand warm water
- Time after start of exposure: 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100, 500 or 2000 mg/kg body weight
- Constant volume or concentration used: no - Duration of exposure:
- 24 h
- Doses:
- 100, 500 or 2000 mg/kg bodyweight
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: repeated obeservation on day of application, and twice a day thereafter (once a day on week ends and holi days), weighing was done right before and 7 and 14 days after application
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, blood parameters (methemoglobin formation) in 100 mg/kg dose group
Results and discussion
- Preliminary study:
- no
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 000 mg/kg bw
- Mortality:
- 2000 mg/kg body weight: all males and four females were euthanized 6 h post-application due to moribund condition
- Clinical signs:
- other: 2000 mg/kg body weight: 30 min post-application: tremor, severe cyanosis, labored breathing, poor condition, face down position, no motor activity, 5 males and 4 females were euthanized due to animal wel
- Gross pathology:
- chromodakryorrhoe, severe cyanosis and dark liquid in the thoracal cavity were observed in moribund euthanized animals, bodies had the typical smell of chlorine, no pathophysiological findings were observed in the animals euthanized after the observation period of 14 d.
Any other information on results incl. tables
no further data
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Remarks:
- Migrated information: T, R24
- Conclusions:
- Like other chloroanilines, the primary toxic effect of 2-chloroaniline is methaemoglobin
formation. Taking into account that humans are much more sensitive to methaemoglobin
producing substances than rats 2-chloroaniline is classified in Toxicity Category III. - Executive summary:
Bomhard, E (1988)
2-chloroaniline was tested for acute dermal toxicity equivalent to EU B.3 on 5 male and 5 female young adult wistar rats per dose level (100, 500 or 2000 mg/kg body weight) .Mortality was only observed in the highest dose level tested. There 9 of 10 animals were euthanized 6 h post-application due to their moribund condition. Following signs of toxicity were observed to decreasing extend in the 2000 and 500 mg/kg body weight dose group: tremor, labored breathing, cyanosis, face down position, poor condition and almost no motor activity. Pathophysiological changes were only evident at the highest dose level and included severe cyanosis and darl liquid in the thoracal cavity. Only the males of the 100 mg/ kg body weight dose group displayed a normal weight gain over time all over surviving test animals showed reduced weight gain one week post-application. Therefore the LD50 was calculated to be 1000 mg/kg body weight. Due to the very severe signs of toxicity the testing laboratory set the testing of additional groups aside. Based on this study 2-chloraniline should be classified as harmful in contact with skin.
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