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EC number: 200-554-5 | CAS number: 63-05-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Dec 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: German standard method "DIN 38412, part 8 (Pseudomonas Zellvermehrungshemm-Test)"
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- 10 mL of nutrient solution were added to the 80 mL test substance solutions. Finally, 10 mL of the inoculum were added. Each test concentration and the control without test compound were set up in duplicate. Additionally, one test vessel without inoculum was used for each test concentration and the control in orderto analyse the substance-dependent turbidity (blank).
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - The microorganisms for the pre-culture were taken from a stock up to seven days old, approx. 7 hours prior to the start of the incubation in the test
- They were kept in a pre-culture nutrient solution; 900 mL sterile distilled water were added; The pre-culture had a turbidity of 0.058 extinction units (equivalent to approximately TE/F 10) on the spectrophotometer
- This pre-culture was incubated for 7 hours at room temperature
- After the incubation period the turbidity of the pre-culture was measured with 0.430 extinction units, and the pre-culture was diluted to 0.214 extinction units (equivalent to approximately TE/F 50) with the pre-culture medium, ready to be used as inoculum - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Hardness:
- No data
- Test temperature:
- 9.8 - 21.6 °C
- pH:
- 8.3
- Dissolved oxygen:
- No data
- Salinity:
- n.a.
- Nominal and measured concentrations:
- The dilutions used were the undiluted saturated solution and 1:10, 1:100, and 1:1000 dilutions thereof. The saturated solution had a concentration of 49.5 mg/L, measured by TOC analysis.
- Details on test conditions:
- - The test vessels were incubated on a shaker at room temperature (approx. 21°C)
- The test organisms were incubated with androstendione for 16 hours
- The test concentrations were prepared by using dilutions of a saturated solution
- The dilutions used were the undiluted saturated solution and 1:10, 1:100, and 1:1000 dilutions thereof
- All groups were set up in triplicate
- Growth was determined by analysing the turbidity of the solutions in a photometer at a wavelength of 436 nm, expressed as TE/F values (turbidity units in relation to a defined formazin solution) - Key result
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 49.5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- Up to a saturated solution, there was no effect on growth of Pseudomona putida. Therefore, the EC10 was determined to be higher than a saturated solution (49.5 mg/L).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Up to a saturated solution, there was no effect of Androstendion on growth of microorganism Pseudomona putida. Therefore, the 16 h-EC10 was determined to be higher than a saturated solution (49.5 mg/L).
- Executive summary:
According to the German standard method "DIN 38412, part 8 (Pseudomonas Zellvermehrungshemm-Test)" the test organisms were incubated with Androstendion for 16 hours. The test concentrations were prepared by using dilutions of a saturated solution. The dilutions used were the undiluted saturated solution and 1:10, 1:100, and 1:1000 dilutions thereof. All groups were set up in triplicate. The saturated solution had a concentration of 49.5 mg/L, measured by TOC analysis. Growth was determined by analysing the turbidity of the solutions in a photometer, expressed as TE/F values (turbidity units in relation to a defined formazin solution).
Up to a saturated solution, there was no effect on growth of Pseudomonas putida. Therefore, the EC10 was determined to be higher than a saturated solution (49.5 mg/L). This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microoragnisms.
Reference
Validity criteria for the measurement of the toxicity to microorganisms:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. If the rate is lower, the test should be repeated with washed activated sludge or with the sludge from another source. The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. | The oxygen uptake was higher | Yes |
The test should be performed at a temperature within the range 20±2°C. | The temperature was 19.8 to 21.6 °C | Yes |
The EC50 and, if possible, 95 % confidence limits, possibly EC20, EC80; possibly NOEC and the used statistical methods, if the EC50 cannot be determined shall be reported. | EC50 is reported | Yes |
Description of key information
Up to a saturated solution, there was no effect of Androstendion on growth of microorganism Pseudomona putida. Therefore, the 16 h-EC10 was determined to be higher than a saturated solution (49.5 mg/L).
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 49.5 mg/L
Additional information
"should read: EC10(16h) > 49.5 mg/L"
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