Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 220-562-2
CAS number: 2814-77-9
This study was performed to investigate the potential of C.I. Pigment
Red 4 to induce gene mutations according to the plate incorporation
assay with rat liver S9 (experiment I), and the pre-incubation test with
hamster liver S9 (experiment II) using the Salmonella typhimurium
strains TA 1535, TA 1537, TA 98 and TA 100 and the Escherichia coli
strain WP2 uvrA.
The assay was performed in two complete experiments with and without
liver microsomal activation and one additional experiment solely with
strain TA 98 in the presence of metabolic activation. The results of the
additional experiment are reported as part of experiment I. Each
concentration, including the controls, was tested in triplicate. The
test item was tested at the following concentrations:
Experiment I (including the additional experiment with strain TA 98): 3;
10; 33; 100; 333; 1000; 2500; and 5000mg/plate.
Experiment II: 33; 100; 333; 1000; 2500; and 5000mg/plate
The plates incubated with the test item showed normal background growth
up to 5000mg/plate with and without metabolic activation in
bath independent experiments.
No toxic effects, evident as a reduction in the number of revertants,
occurred in the test groups with and without metabolic activation. No
substantial increase in revertant colony numbers was observed following
treatment with C.I Pigment Red 4 at any dose level, neither in the
presence nor absence of metabolic activation (S9 mix) in strains TA
1535, TA 1537, TA 100, and WP2 uvrA. However, a substantial increase was
observed in strain TA 98 in the presence of metabolic activation in both
independent experiments. The required threshold of two times the
mutation frequency of the corresponding solvent control was well
exceeded at concentrations as low as 3mg/plate in
experiment I and 33mg/plate in experiment II. To verify the
results of experiment I, an additional plate incorporation test was
performed with strain TA 98 in the presence of metabolic activation. In
this additional experiment the result of the first experiment was
Appropriate reference mutagens were used as positive controls and showed
a distinct increase of induced revertant colonies.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again