Methanesulphonyl chloride

Endpoint summary

Additional information

In vitro study

In an test performed according to the OECD guidelines #471 and GLP (Dawkes, 1997), which included treatments at concentrations up to 10000 µg/plate, methanesulfonyl chloride (purity 99.8%) was assayed for mutation in five histidine-requiring strains (TA98, TA100, TA1535, TA1537 and TA102) of Salmonella typhimurium, both in the absence and in the presence of metabolic activation by an Aroclor 1254-induced rat liver post-mitochondrial fraction (S-9). Methanesulfonyl chloride induced mutagenic activity on TA 1535 and exhibited evidence of weak mutagenic activity in TA 100 strains, both with and without metabolic activation.

In an test performed according to the OECD guidelines #471 and GLP (Dawkes, 1998), which included treatments at concentrations up to 10000 µg/plate, methanesulfonyl chloride (purity 99.8%) was assayed for mutation in two histidine-requiring strains (TA100 and TA1535) ofSalmonella typhimurium, both in the absence and in the presence of metabolic activation by an Aroclor 1254-induced rat liver post-mitochondrial fraction (S-9). Methane sulfonyl chloride induced mutagenic activity on TA 1535, both with and without metabolic activation.

Methanesulfonyl chloride (purity 99.85%) was tested in an in vitro cytogenetics assay performed according to the OECD guidelin #473 and GLP (Marshall, 1997). Treatments of duplicate human lymphocyte cultures were performed both in the absence and presence of metabolic activation by a rat liver post-mitochondrial fraction (S-9) from Aroclor 1254 induced animals. The test article was dissolved in acetone and the highest dose level used, 1150 µg/mL, was equivalent to a concentration of 10 mM. Methanesulfonyl chloride induced chromosome aberrations in cultured human peripheral blood lymphocytes. The effect was seen following treatment in both the absence and presence of S-9.

In vivo study

The potential of methanesulfonyl chloride (purity 99.9%) to induce structural or numerical damage in bone marrow cells of mice was evaluated a study performed according to the OECD guideline #474 and GLP (Haddouk, 2003).

Three groups of five male and five female mice were given intraperitoneal administrations (10 ml/kg) of methanesulfonyl chloride at dose-levels of 0 (paraffin oil), 7.5,15 and 30 mg/kg/day, over a 2-day period. One group of five males and five females received the positive control test item (cyclophosphamide) once by oral route at the dose-level of 50 mg/kg.

Animal were sacrificed 24 hours after the last administration, and bone marrow smears examined for the presence of micronuclei in 2000 polychromatic erythrocytes per mouse and for the ratio PCE/NCE.

For both males and females, the mean values of MPE as well as the PE/NE ratio in the groups treated with the test item, were equivalent to those of the vehicle group. Methanesulfonyl chloride was concluded to be negative in the mouse micronucleus assay.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

Not classified for germ cells mutation.