1,2,4-Benzenetricarboxylic acid, mixed lauryl and octyl triesters

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2014-04-19 - 2014-12-04

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

N/A

Analytical monitoring:

yes

Details on sampling:

- Sampling method: For the analysis of the actual test substance concentrations, 30 mL of test solution was taken from each test vessel at the beginning and at the end of the test.
- Sample storage conditions before analysis: directly analyzed without storage

Vehicle:

no

Details on test solutions:

500.8 mg test substance was added in 5 L test water and a nominal concentration of 100 mg/Lof the test substance was prepared and stirred (via magnetic stirrer) in the dark at 500 rpm followed by filtration using a 0.45 µm nitrocellulose membrane to make a saturated solution of the test substance in the test water.

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

TEST ORGANISM
- Common name: Minnow
- Strain: Gobiocypris rarus
- Source: Bioassay and Safety Assessment Lab, Shanghai Academy of Public Measurement
- Age at study initiation (mean and range, SD): not mentioned
- Feeding during test: none


ACCLIMATION
- Acclimation period: more than 14 days
- Acclimation conditions (same as test or not): The test water used was tapwater dechlorinated by activated carbon and sterilyzed by UV lamp.
Temperature 21-25 °C and constant with a range of 2 °C. Oxygen concentration: > 80 % of air saturation value (ASV), Photoperiod: Light : Dark = 12 hours :12 hours.
- Length was 2.2 ± 0.1 cm (Mean ± SD) and weight was 0.18 ± 0.03 g (Mean ± SD) at the end of the test.
- Feeding frequency: daily until 24 hours before the test was started
- Health during acclimation (any mortality observed): No diseases were observed during the acclimation period. No mortality was present the 7 days before the start of the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none

Hardness:

145 mg CaCO3/L

Test temperature:

23.8 - 24.3 °C

pH:

6 - 8.5

Dissolved oxygen:

75.3 - 91.8 %

Salinity:

N/A

Conductivity:

N/A

Nominal and measured concentrations:

Nominal: 100 mg/L WAF
Measured: below LOD of 0.274 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 L glass tanks containing 4 L test solution.
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.5 g/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water, dechlorinated by activated carbon and sterilized by UV lamp. The water was aerated continously over 24 h.


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 hours light, 12 hours darkness
- Light intensity: not mentioned


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality after 24, 48, 72 and 96 hours; pH, temperature, dissolved Oxygen at the same time.
At the end of test, the body length and body weight of the test fish of the control were measured.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test, test concentration limited by test item's water solubility)
- Range finding study: The log Pow of the test substance was estimated to 13.4, it is considered to have an adsorption. Therefore, the test vessels and membranes were pretreated (supersaturated) before the test. 501.3 mg test substance was prepared and stirred (via magnetic stirrer) in the dark for 72 h at 500 rpm followed by filtration using a 0.45 µm nitrocellulose membrane to make a saturated solution of the test substance in the test water. One concentration of the saturated solution (the measured concentration was below the LOD of 0.274 mg/L) was used. In this test, a blank control of test water was used. Five fishes were observed and dead fish were recorded at 0, 24, 48, 72 and 96 h after test initiation. In the range-finding test, there were no mortalities and abnormalities observed in the fish of the control group and the treatment group during the test.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: no mortality at limit of water solubility

Details on results:

N/A

Results with reference substance (positive control):

no reference substance tested

Reported statistics and error estimates:

no statisitcs performed, as no mortality occured

Sublethal observations / clinical signs:

The mortatlities and abnormalities in limit test (10 fish in each concentration):

Group	Duration (h)	Mortalities	Cumulative Mortalities	Abnormalities
Blank control	0	0	0	none
	24	0	0	none
	48	0	0	none
	72	0	0	none
	96	0	0	none
Treatment Group (Saturated solution)	0	0	0	none
	24	0	0	none
	48	0	0	none
	72	0	0	none
	96	0	0	none

Validity criteria fulfilled:

yes

Conclusions:

There was no acute toxicity of the saturated solution in the limit test. The test results showed that the 96 h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution under the test conditions. Not toxic under test conditions in the range of water solubility.

Executive summary:

According to "Ministry of environmental protection of the people's Republic of China, the guidelines for the testing of chemicals-effects on biotic system, 2013, 203 Fish, OECD 203, the acute toxicity of the test substance to Gobiocypris rarus was determined by a static test. A nominal concentration of 100 mg/L of the test substance was prepared and stirred (via magnetic stirrer) in the dark for 72 h at 500 rpm followed by filtration using 0.45 µm nitrocellulose membrane to make a saturated solution of the test substance in the test water (referred to as "saturated solution" in this report). The octanol/water partition coefficient (log Pow) of the test substance was estimated to 13.4, it is considered to have an adsorption. Therefore, the test vessels and membranes were pretreated (supersaturated) before the test. Based on the results of the range-finding test, the saturated solution was used to perform the limit test with a blank control. The test duration was 96h. No mortality occurred in the control during the test and the dissolved oxygen concentration was greater than 60 % at the air saturation value throughout the test duration. Hence, the test was considered to be valid.

During the test, the measured concentrations of the test substance analyzed by Gas Chromatograph (GC) were below the limit of detection (LOD, LOD = 0.274 mg/L). There were no mortalities and abnormalities observed in the treatment group during the test period. There was no acute toxicity of the saturated solution in the limit test. The test results showed that the 96 h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution (which measured concentration was below LOD, LOD = 0.274 mg/L) under the test conditions.

Description of key information

There was no acute toxicity of the saturated solution in the limit test. The test results showed that the 96 h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution under the test conditions. Not toxic under test conditions in the range of water solubility.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L

Additional information

According to "Ministry of environmental protection of the people's Republic of China, the guidelines for the testing of chemicals-effects on biotic system, 2013, 203 Fish, OECD 203, the acute toxicity of the test substance to Gobiocypris rarus was determined by a static test. A nominal concentration of 100 mg/L of the test substance was prepared and stirred (via magnetic stirrer) in the dark for 72 h at 500 rpm followed by filtration using 0.45 µm nitrocellulose membrane to make a saturated solution of the test substance in the test water (referred to as "saturated solution" in this report). The octanol/water partition coefficient (log POW) of the test substance was estimated to 13.4, it is considered to have an adsorption. Therefore, the test vessels and membranes were pretreated (supersaturated) before the test. Based on the results of the range-finding test, the saturated solution was used to perform the limit test with a blank control. The test duration was 96h. No mortality occured in the control during the test and the dissolved oxygen concentration was greater than 60 % at the air saturation value throughout the test duration. Hence, the test was considered to be valid.

During the test, the measured concentrations of the test substance analyzed by Gas Chromatograph (GC) were below the limit of detection (LOD, LOD = 0.274 mg/L). There were no mortalities and abnormalities observed in the treatment group during the test period. There was no acute toxicity of the saturated solution in the limit test. The test results showed that the 96 h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution (which measured concentration was below LOD, LOD = 0.274 mg/L) under the test conditions.