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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Description of key information

In the key study, ready biodegradability was tested according to the guideline AFNOR T 90-312 [equivalent or similar to the DOC Die-Away test (OECD 301 A, EC C.4-A)] in 1988 by Atochem (Boutonnet JC).  The initial test substance concentration was 57.5 mg/L, and the test was performed with a mixture of activated sludge from the wastewater treatment plant at Versailles and water from the river Seine. DOC removal was monitored over a time period of 28 days, and the test substance was completely degraded after 14 days. From the degradation curve, 10% degradation of the test substance was reached after ~1.5 days, and 70% after 10 days, showing that the 10-day window criterion was met. The reference substance, sodium benzoate, achieved 94.4% DOC removal after 4 days and was totally degraded after 14 days.  All conditions for validity were met (differences between test substance duplicates were <20%, the reference substance exceeded 70% degradation within 14 days, and a toxicity control showed that the substance was not inhibitory to the inoculum). The substance is considered to be readily biodegradable under the conditions of the test.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information