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EC number: 200-865-6 | CAS number: 75-36-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No data on GLP and no guideline followed. However, the method is similar to the guideline OECD 471.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
- Principles of method if other than guideline:
- Ames test, preincubation assay. The test chemical , Salmonella culture, and S-9 mix or buffer were incubated at 37 ºC, without shaking, for 20 min. The top agar was added and the contents of the tubes were mixed and poured onto the surface of petri dishes containing Vogel-Bonner medium. The histidine-independent (his+) colonies arising on these plates were counted following two days incubation at 37°C.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Acetyl chloride
- EC Number:
- 200-865-6
- EC Name:
- Acetyl chloride
- Cas Number:
- 75-36-5
- Molecular formula:
- C2H3ClO
- IUPAC Name:
- acetyl chloride
- Details on test material:
- - Name of test material (as cited in study report): Acetyl chloride
- Label purity: 99+%
- Analyzed purity: 98%
- Source: Aldrich Chemical
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA97/TA100/TA1535/TA1537
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix: the S9 fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were prepared. The S9 mixes contained either 10% or 30% S9.
- Test concentrations with justification for top dose:
- 0, 33, 100, 333, 1000, 3333 and 6666 μg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: acetone
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without metabolic activation for TA1535 and TA100
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without metabolic activation for TA97 and TA1537
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- with metabolic activation for all strains
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
NUMBER OF REPLICATIONS: Three plates
DETERMINATION OF CYTOTOXICITY: The chemical was tested initially in a toxicity assay to determine the appropriate dose range for the mutagenicity assay. The toxicity assay was performed using TA100 or the system developed by Waleh et al. (1982). Toxic concentrations were those that produced a decrease in the number of his+ colonies, or a clearing in the density of the background lawn, or both. - Evaluation criteria:
- A chemical was judged mutagenic or weakly mutagenic if it produced a reproducible dose-related reponse over the solvent control in replicate trials.
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA97/100/1535/1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (at the highest concentration tested)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: Salmonella typhimurium TA97/100/1535/1537
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Acetyl chloride is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose level investigated.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Acetyl chloride is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose level investigated. - Executive summary:
The Ames test using the preincubation assay was conducted. The test chemical , Salmonella culture, and S-9 mix or buffer were incubated at 37 ºC, without shaking, for 20 min. The top agar was added and the contents of the tubes were mixed and poured onto the surface of petri dishes containing Vogel-Bonner medium. The histidine-independent (his+) colonies arising on these plates were counted following two days incubation at 37°C. Acetyl chloride is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose level investigated.
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