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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No data on GLP and no guideline followed. However, the method is similar to the guideline OECD 471.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1988

Materials and methods

Principles of method if other than guideline:
Ames test, preincubation assay. The test chemical , Salmonella culture, and S-9 mix or buffer were incubated at 37 ºC, without shaking, for 20 min. The top agar was added and the contents of the tubes were mixed and poured onto the surface of petri dishes containing Vogel-Bonner medium. The histidine-independent (his+) colonies arising on these plates were counted following two days incubation at 37°C.
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetyl chloride
EC Number:
200-865-6
EC Name:
Acetyl chloride
Cas Number:
75-36-5
Molecular formula:
C2H3ClO
IUPAC Name:
acetyl chloride
Details on test material:
- Name of test material (as cited in study report): Acetyl chloride
- Label purity: 99+%
- Analyzed purity: 98%
- Source: Aldrich Chemical

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA97/TA100/TA1535/TA1537
Metabolic activation:
with and without
Metabolic activation system:
S9 mix: the S9 fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were prepared. The S9 mixes contained either 10% or 30% S9.
Test concentrations with justification for top dose:
0, 33, 100, 333, 1000, 3333 and 6666 μg/plate
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: acetone
Controlsopen allclose all
Negative solvent / vehicle controls:
yes
Positive controls:
yes
Positive control substance:
sodium azide
Remarks:
without metabolic activation for TA1535 and TA100
Negative solvent / vehicle controls:
yes
Positive controls:
yes
Positive control substance:
9-aminoacridine
Remarks:
without metabolic activation for TA97 and TA1537
Negative solvent / vehicle controls:
yes
Positive controls:
yes
Positive control substance:
other: 2-aminoanthracene
Remarks:
with metabolic activation for all strains
Details on test system and experimental conditions:
METHOD OF APPLICATION: preincubation


NUMBER OF REPLICATIONS: Three plates


DETERMINATION OF CYTOTOXICITY: The chemical was tested initially in a toxicity assay to determine the appropriate dose range for the mutagenicity assay. The toxicity assay was performed using TA100 or the system developed by Waleh et al. (1982). Toxic concentrations were those that produced a decrease in the number of his+ colonies, or a clearing in the density of the background lawn, or both.
Evaluation criteria:
A chemical was judged mutagenic or weakly mutagenic if it produced a reproducible dose-related reponse over the solvent control in replicate trials.

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium TA97/100/1535/1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(at the highest concentration tested)
Vehicle controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: other: Salmonella typhimurium TA97/100/1535/1537
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Acetyl chloride is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose level investigated.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

Acetyl chloride is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose level investigated.
Executive summary:

The Ames test using the preincubation assay was conducted. The test chemical , Salmonella culture, and S-9 mix or buffer were incubated at 37 ºC, without shaking, for 20 min. The top agar was added and the contents of the tubes were mixed and poured onto the surface of petri dishes containing Vogel-Bonner medium. The histidine-independent (his+) colonies arising on these plates were counted following two days incubation at 37°C. Acetyl chloride is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose level investigated.