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EC number: 229-194-7
CAS number: 6425-39-4
The key study was performed equivalent to OECD guideline 301C under GLP conditions. Only 1 to 4 % degradation was observed with the test substance after 28 days, depending on the parameter monitored (BOD, TOC removal, or test material analysis using GC), whereas the reference substance (aniline) showed up to 77 % degradation after 28 days. Up to 50 % of a control substance was degraded after 7 days in the presence of the test substance. Therefore, it can be concluded that the test results are valid. In conclusion, a 28-day modified MITI-test showed that the substance is not readily biodegradable.
Two studies describing a ready biodegradability test were identified.
The study of BASF (1997) describes a 28 -days DOC die-away test, and the
study of Kurume Research Laboratories (1993) concerns a 28 -days
modified MITI test. However, the BASF study also claims to be performed
according to OECD 302 B (Zahn-Wellens test), which is an inherent
biodegradability test, and according to ISO 7827, which is an ultimate
biodegradation guideline. Both studies, although using different
methods, come to the same conclusion that the test substance is not
biodegradable under test conditions. In the BASF study 0 -10 %
biodegradation was observed, whereas the study of Kurume Research
Laboratories showed 1 to 4 % degradation. Aniline was used as reference
substance in either study. As the study of Kurume Research Laboratories
is performed according to GLP and allowed a more thorough evaluation of
the performed test, this study was rated K1 and assigned as key study.
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