Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
year of publication: 1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Sufficently documented literature data

Data source

Reference
Reference Type:
publication
Title:
Salmonella Mutagenicity Tests: V. Results from the testing of 311 chemicals
Author:
Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K
Year:
1992
Bibliographic source:
Env. Molecular Mutagenisis 19:2-141

Materials and methods

Principles of method if other than guideline:
Bacterial reverse mutation assay (Ames test), preincubation assay
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chlorobenzaldehyde
EC Number:
201-956-3
EC Name:
2-chlorobenzaldehyde
Cas Number:
89-98-5
Molecular formula:
C7H5ClO
IUPAC Name:
2-chlorobenzaldehyde
Details on test material:
- Name of test material (as cited in study report): 2-chlorobenzaldehyde
- Analytical purity: 95.1%
- Impurities (identity and concentrations): no data

Method

Species / strain
Species / strain / cell type:
S. typhimurium, other: TA 97, TA 98, TA 100, TA 1535, TA 1537
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9-mix
Test concentrations with justification for top dose:
0 (control), 3.3, 10.0, 33.0, 100.0, 200.0, 333.0, 500.0, 666.0 µg/plate
Vehicle / solvent:
DMSO
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
Remarks:
DMSO
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: without metabolic activation: sodium azide (TA1535 and TA100), 9-aminoacridine (TA97 and TA1537), 4-nitro-o-phenylenediamine (TA 98 and 1538); with metabolic activation: 2-aminoanthracene (all strains)
Details on test system and experimental conditions:
20 min preincubation procedure

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA 97, TA 98, TA 100, TA 1535, TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
It was stated in the text, that maximum concentrations of 10000 µg/plate are only tested if substances were not toxic. As 2-chlorobenzaldehyde was only tested up to 666 µg/plate it can be concluded, that it was toxic at higher concentrations. But, no results of the testing for cytoxicity were presented in the publication.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

2-Chlorobenzaldehyde was not mutagenic under the conditions tested.
Executive summary:

2-Chlorobenzaldehyde was not mutagenic in the bacterial reverse mutation assay (Ames test, test strains: Salmonella typhimurium TA 97, TA 98, TA 100, TA 1535, TA 1537) with and without metabolic activation (S9 -mix).

This well documented and reliable (RL2) study has been selected as key study for the evaluation of gene mutations.