1-isopropyl-4-methylcyclohexane

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01/03 1989 to 15/03 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: conform the guideline, non-GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

p-menthane

IUPAC Name:

p-menthane

Constituent 2

Reference substance name:

1-isopropyl-4-methylcyclohexane

EC Number:

202-790-4

EC Name:

1-isopropyl-4-methylcyclohexane

Cas Number:

99-82-1

Molecular formula:

C10H20

IUPAC Name:

1-methyl-4-(propan-2-yl)cyclohexane

Details on test material:

- Name of test material (as cited in study report): p-menthane
- Physical state: liquid

Test animals

Species:

rabbit

Strain:

other: white russian

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. Karl Tomae GmbH Biberach
- Sex: male
- Weight at study initiation: 2.2 - 2.4 kg
- Housing: individual
- Diet : K4 Alleindiät für Kaninchen, Ssniff Spezialfütter GmbH Soest, ad libitum
- Water: ad libitum
- Acclimation period: 14 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ±1
- Humidity (%): 60 ±5
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: 01 March 1989 To: 15 March 1989

Test system

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

0.5 cm3

Duration of treatment / exposure:

4 hours

Observation period:

scored at 1, 24, 48 and 72 hours and after 6, 8, 10 and 14 days

Number of animals:

3

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Remarks:

animals 1 - 3

Time point:

24 h

Score:

2

Max. score:

3

Reversibility:

not reversible

Remarks on result:

other: scabs from day 6 onwards

Results 2

Irritation parameter:

edema score

Basis:

mean

Remarks:

animals 1 - 3

Time point:

24 h

Score:

4

Max. score:

4

Reversibility:

not reversible

Results 3

Irritation parameter:

erythema score

Basis:

mean

Remarks:

animals 1 - 3

Time point:

48 h

Score:

2

Max. score:

3

Reversibility:

not reversible

Remarks on result:

other: scabs from day 8 onwards

Results 4

Irritation parameter:

edema score

Basis:

mean

Remarks:

animals 1 - 3

Time point:

48 h

Score:

2

Max. score:

4

Reversibility:

not reversible

Results 5

Irritation parameter:

erythema score

Basis:

mean

Remarks:

animals 1 - 3

Time point:

72 h

Score:

2

Max. score:

3

Reversibility:

not reversible

Remarks on result:

other: scabs from day 8 onwards

Results 6

Irritation parameter:

edema score

Basis:

mean

Remarks:

animals 1 - 3

Time point:

72 h

Score:

1.7

Max. score:

4

Reversibility:

not reversible

Any other information on results incl. tables

 

	1h		24 h		48 h		72 h		6 d		8 d		10 d		14 d
	E	O	E	O	E	O	E	O	E	O	E	O	E	O	E	O
Animal 1	3	4	2	4	2	2	2	2	3*	3	scabs		scabs		Loose scabs
Animal 2	2	4	2	4	2	2	2	2	2#	2	scabs		scabs		Loose scabs
Animal 3	2	4	2	4	2	2	2	1	3*	3	scabs		scabs		Loose scabs

*Redbraun discoloration and dryness of the skin

#Redbraun discoloration of the skin

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

The test substance is considered moderately irritant to the skin

Executive summary:

Dermal application of the test substance to rabbits resulted in erythema and necrosis after 4 hours exposure. The test substance is considered irritant to the skin.