2-butyl-2-ethylpropanediol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

acute, 48 hours, 28 to 30 June 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline and GLP-compliant study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-Butyl-2-ethyl-1,3-propanediol

IUPAC Name:

2-Butyl-2-ethyl-1,3-propanediol

Constituent 2

Reference substance name:

2-butyl-2-ethylpropanediol

EC Number:

204-111-7

EC Name:

2-butyl-2-ethylpropanediol

Cas Number:

115-84-4

Molecular formula:

C9H20O2

IUPAC Name:

2-butyl-2-ethylpropane-1,3-diol

Details on test material:

- Name of test material (as cited in study report): BEPD
- Physical state: colourless solid
- Analytical purity: 99.5%
- Lot/batch No.: HRC accession number 20.4.95/1
- Expiration date of the lot/batch: 31 December 1995.
- Storage condition of test material: in darkness at room temperature (ca. 20 degrees C).

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken at 0 hours (fresh media) and 48 hours (expired media).

Test solutions

Vehicle:

no

Details on test solutions:

The test material was dispersed in test water with the aid of ultrasonic disruption for 30 minutes.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test organism:
- Common name: Daphnia magna
- Source: laboratory culture
- Age at study initiation (mean and range, SD):

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

Not applicable

Test conditions

Test temperature:

21 - 22 °C

pH:

7.9 - 8.3

Dissolved oxygen:

7.6 - 8.3 mg/L

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal: 0 (control), 100 mg/L
Measured: 0 (control), 95 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Any other information on results incl. tables

The results of the definitive test are compiled below:

Nominal BEPD concentration (mg/L)	Mean BEPD concentration (mg/L)				Mean immobilisation after 48 hours (%)
	0 hours	48 hours (E)	Mean	Mean as % of nominal
Control	ND	ND	-	-	0
100	96.5	93.44	95	95	0

ND: None detected

E: Expired media

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour acute toxicity of BEPD toDaphnia magna wascarried out in a static system according to OECD 202 and EU guidelines. First instar daphnids were exposed over a 48 hour period to BEPD at nominal concentrations of 0 (control) and 100 mg/L. Duplicate, loosely covered vessels, each containing 10 daphnids in 200 ml medium were allocated to each treatment. Concentration of BEPD was verified analytically at test initiation and termination and mean measured concentration was 95 mg/L. Individual pH, temperature and dissolved oxygen values remained within acceptable limits throughout the duration of the study. The 48-hour EC50 was determined to be >100 mg BEPD/L (nominal).

Executive summary:

The 48 hour acute toxicity of BEPD to Daphnia magna was carried out in a static system according to OECD 202 and EU guidelines. First instar daphnids were exposed over a 48 hour period to BEPD at nominal concentrations of 0 (control) and 100 mg/L. Duplicate, loosely covered vessels, each containing 10 daphnids in 200 ml medium were allocated to each treatment. Concentration of BEPD was verified analytically at test initiation and termination and mean measured concentration was 95 mg/L. Individual pH, temperature and dissolved oxygen values remained within acceptable limits throughout the duration of the study. The 48-hour EC50 was determined to be >100 mg BEPD/L (nominal).