Registration Dossier
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EC number: 613-901-4 | CAS number: 6615-00-5
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
EyC50 (algae; yield)=72 mg/L (95% C.I. 52-110 mg/L), 72h, freshwater, OECD 201, Soucy 2012
ErC50 (algae; growth rate)= >100 mg/L (no concentration tested resulted in ≥ 50% inhibition of growth rate, the 72-hour ErC50 was empirically estimated, therefore 95% CI could not be determined), 72h, freshwater, OECD 201, Soucy 2012
NOEC (algae; growth rate) = 26 mg/l 72h, freshwater, OECD 201, 2012
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 26 mg/L
Additional information
The influence of the test material on the growth of the green algal species Pseudokircheriella subcapitata, was investigated in a 72 hour static test according to the OECD Guideline No. 201. The nominal test concentrations were 6.3, 13, 25, 50 and 100 mg/l in parallel with a control. Mean measured concentrations were all 100% of nominal concentrations and defined the treatment levels tested as 6.4, 13, 26, 51 and 100 mg/l. The algal population grew well, resulting in an average of 1029200 cells/mL in the control after 72 hours. No effects on algal health were noted during the test. Exposure of algae to the test material for 72 hours resulted in a median effective concentration (EyC50) of 72 mg/l (95% confidence interval = 52 to 110 mg/l) when determined using the number of cells/mL, and >100 mg/L when determined using the average specific growth rate (since no concentration tested resulted in ≥ 50% inhibition of growth rate, the 72-hour ErC50 was empirically estimated, therefore 95% CI could not be determined). A NOEC value for growth rate and cell number was determined to be 26 mg/l.
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