2-aminobutan-1-ol

Additional toxicological data

Administrative data

Endpoint:

additional toxicological information

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Published data not conforming to a standard guideline but still considered tobe reliable

Data source

Reference

Reference Type:

publication

Title:

Effects of Analogues of Ethanolamine and Choline on Phospholipid Metabolism in Rat Hepatocytes

Author:

Bjorn Akesson

Year:

1977

Bibliographic source:

Biochem. J. (1977) 168, 401-408

Materials and methods

Type of study / information:

Study assessing the incorporation of various amino alcohol substances into the phospholipids of isolated rat hepatocytes

Test guideline

Qualifier:

no guideline followed

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-aminobutan-1-ol

EC Number:

202-488-2

EC Name:

2-aminobutan-1-ol

Cas Number:

96-20-8

Molecular formula:

C4H11NO

IUPAC Name:

2-aminobutan-1-ol

Applicant's summary and conclusion

Conclusions:

2-aminobutanol appears to inhibit the incorporation of ethanolamine into phosphatidyl ethanolamine and the incorporation of choline into phosphatidyl choline. 2-amino-2-methylpropanol appeared to inhibit choline incorporation into phosphatidyl choline but not ethanolamine incorporation. This is contrary to other published work (Longmore and Mulford 1962) which dosed in vivo to rats and indicated the contrary effect, i.e. inhibition of ethanolamine incorporation.

Executive summary:

Analogues of ethanolamine and choline were incubated with different labelled precursors

of phospholipids and isolated hepatocytes and the effects on phospholipid

synthesis were studied. 2. 2-Aminopropan-1-ol and 2-aminobutan-1-ol were the most

efficient inhibitors of ['4C]ethanolamine incorporation into phospholipids, whereas the

incorporation of [3H]choline was inhibited most extensively by NN-diethylethanolamine

and NN-dimethylethanolamine. 3. When the analogues were incubated with [3H]glycerol

and hepatocytes, the appearance of 3H in unnatural phospholipids indicated that they

were incorporated, at least in part, via CDP-derivatives. The distribution of [3H]glycerol

among molecular species of phospholipids containing 2-aminopropan-1-ol and 1-aminopropan-

2-ol was the same as in phosphatidylethanolamine. In other phospholipid analogues

the distribution of 3H was more similar to that in phosphatidylcholine. 4. NNDiethylethanolamine

stimulated both the conversion of phosphatidylethanolamine into

phosphatidylcholine and the incorporation of [Me-14C]methionine into phospholipids.

Other N-alkyl- or NN-dialkyl-ethanolamines also stimulated [14C]methionine incorporation,

but inhibited the conversion of phosphatidylethanolamine into phosphatidylcholine.

This indicates that phosphatidyl-NN-diethylethanolamine is a poor methyl

acceptor, in contrast with other N-alkylated phosphatidylethanolamines. 5. These results

on the regulation of phospholipid metabolism in intact cells are discussed with respect to

the possible control points. They also provide guidelines for future experiments on the

manipulation of phospholipid polar-headgroup composition in primary cultures of

hepatocytes.