Bis(tributyltin) oxide

Toxicity to soil microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

January-February 1968

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

other: DIN 52 176

Deviations:

yes

Remarks:

(Impregnation of pine block test media diverged from the standard)

Principles of method if other than guideline:

Impregnation of pine sapwood block involved evacuating air from the blocks in the dry state followed by introduction of the preservative solutions under vacuum

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Details on preparation and application of test substrate:

Solvent used was chloroform complying with DAB 6 (German Pharmacopoeia 6th edition).

Test organisms (inoculum):

soil

Total exposure duration:

12 wk

Moisture:

Not reported

Nominal and measured concentrations:

For tests without leaching: 0.001, 0.0025, 0.0063, 0.016, 0.04, 0.1 %
For tests with leaching: 0.0025, 0.0063, 0.016, 0.04, 0.25 %

The toxic limits determined in the tests are collated in the tables given below. The preservative loading is expressed in terns of the mean values from the 4 tested replicates. A deciding factor for the determination of the toxic limits was a weight loss of <3% for those blocks in every test series which were most strongly attacked.

Toxic Limits for Basidiomycete
Tri-n-butyltin oxide
Test fungi	Concentration (%)	Preservartive loading (kg/m³)
Without Leaching
Coniophora cerebella	0.04-0.10	0.344-0.704
Poria vaporaria	0.0025-0.0063	0.022-0.058
Lentinus lepideus	0.0063-0.0160	0.054-0.144
With Leaching
Coniophora cerebella	0.10-0.25	0.681-2.178
Poria vaporaria	0.0025-0.0063	0.021-0.055
Lentinus lepideus	0.0063-0.0160	0.056-0.140
Toxic Limits for Soft Rot Fungi
Tri-n-butyltin oxide
Test fungi	Concentration (%)	Preservartive loading (kg/m³)
Without Leaching
Chaetomium globosum	>0.10	>0.485
Paecilomyces spp.	0.04-0.10	0.269-0.572
Soil burial test	>0.10	>0.577
With Leaching
Chaetomium globosum	0.10-0.25	0.643-1.562
Paecilomyces spp.	0.10-0.25	0.438-1.695
Soil burial test	>0.25	>1.662

Validity criteria fulfilled:

not specified

Conclusions:

Toxic limits for basidiomycetes exposed to tri-n-butyltin oxide without leaching ranged from 0.04% to 0.1% for Coniphora cerebella; 0.0025% to 0.0063% for Poria vaporaria; and 0.0063% to 0.016% for Lentinus lepideus. Toxic limits for basidiomycetes exposed to tri-n-butyltin oxide with leaching ranged from 0.1% to 0.25% for Coniphora cerebella; 0.0025% to 0.0063% for Poria vaporaria; and 0.0063% to 0.016% for Lentinus lepideus.

Toxic limits for soft rot fungi exposed to tri-n-butyltin oxide without leaching was greater than 0.1% for Chaetomium globosus; 0.04% to 0.1% for Paecilomyces sp., and greater than 0.1% for the soil burial test. Toxic limits for soft rot fungi exposed to tri-n-butyltin oxide with leaching ranged from 0.1% to 0.25% for Chaetomium globosus; 0.1% to 0.25% for Paecilomyces sp., and much greater than 0.25% for the soil burial test.

Executive summary:

Toxic limits for basidiomycetes exposed to tri-n-butyltin oxide without leaching ranged from 0.04% to 0.1% for Coniphora cerebella; 0.0025% to 0.0063% for Poria vaporaria; and 0.0063% to 0.016% for Lentinus lepideus. Toxic limits for basidiomycetes exposed to tri-n-butyltin oxide with leaching ranged from 0.1% to 0.25% for Coniphora cerebella; 0.0025% to 0.0063% for Poria vaporaria; and 0.0063% to 0.016% for Lentinus lepideus.

Toxic limits for soft rot fungi exposed to tri-n-butyltin oxide without leaching was greater than 0.1% for Chaetomium globosus; 0.04% to 0.1% for Paecilomyces sp., and greater than 0.1% for the soil burial test. Toxic limits for soft rot fungi exposed to tri-n-butyltin oxide with leaching ranged from 0.1% to 0.25% for Chaetomium globosus; 0.1% to 0.25% for Paecilomyces sp., and much greater than 0.25% for the soil burial test.

Leaching appeared to decrease the toxicity of tri-n-butyltin oxide to various fungi. Poria vaporaria was the most sensitive species tested.

Description of key information

Toxic limits for basidiomycetes exposed to tri-n-butyltin oxide without leaching ranged from 0.04% to 0.1% for Coniphora cerebella; 0.0025% to 0.0063% for Poria vaporaria; and 0.0063% to 0.016% for Lentinus lepideus. Toxic limits for basidiomycetes exposed to tri-n-butyltin oxide with leaching ranged from 0.1% to 0.25% for Coniphora cerebella; 0.0025% to 0.0063% for Poria vaporaria; and 0.0063% to 0.016% for Lentinus lepideus.

Toxic limits for soft rot fungi exposed to tri-n-butyltin oxide without leaching was greater than 0.1% for Chaetomium globosus; 0.04% to 0.1% for Paecilomyces sp., and greater than 0.1% for the soil burial test. Toxic limits for soft rot fungi exposed to tri-n-butyltin oxide with leaching ranged from 0.1% to 0.25% for Chaetomium globosus; 0.1% to 0.25% for Paecilomyces sp., and much greater than 0.25% for the soil burial test.

Leaching appeared to decrease the toxicity of tri-n-butyltin oxide to various fungi. Poria vaporaria was the most sensitive species tested.

Additional information

The study Liese, W., & Liese, Willeitner, H., H. (1969) was provided for informative purposes only. The study was limited in its level of reporting and was not performed to a standardised guideline or in compliance with GLP. The study was assigned a reliability score of 4.