Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
at t=0h, t=24h and 72h

Test solutions

Vehicle:
yes
Details on test solutions:
4.6, 10, 22, 46 and 100% of a 5µm filtrate prepared at 100mg/l

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Post exposure observation period:
at t=0h, t=24h and t=72h

Test conditions

Hardness:
24mg CaCO3/l
Test temperature:
23.9 - 24.2°C
pH:
8.5 - 9.0
Nominal and measured concentrations:
Measured concentrations: 0.031mg/l, 0.077mg/l, 0.22mg/l, 0.52mg/l and 1.4mg/l

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.03 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
other: growth rate + cell growth
Remarks on result:
other: No EC-values determined, since the apparent effect on cell growth was not considered to be biologically significant, while the effect on growth rate was smaller than 10%
Details on results:
Due to the very low solubility of TRUMP IR DYE in water (0.03mg/l), concentration levels that might be toxic for algae could not be reached. Both for growth inhibition and growth rate reduction, the EC50 is higher than the maximum solubility of TRUMP IR DYE in test medium.

Applicant's summary and conclusion

Validity criteria fulfilled:
no
Remarks:
Exposure concentrations of solution without algae did not remain above 80% relative to initial concentrations (could not be prevented, as no intermittent renewal allowed; decrease of test substance not due to adsorption to algae but due to low solubility)
Conclusions:
not toxic to algae up to water solubility