Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
2.5mmol/l as CaCO3
Test temperature:
21°C
pH:
7.8 - 8.0
Dissolved oxygen:
8.4 - 8.5mg/l
Salinity:
Analytical grade salts were dissolved in purified water to obtain the following nominal concentrations:
- CaCl2.2H2O: 2.0mmol/l
- MgSO4.4H2O: 0.5mmol/l
- NaHCO3: 0.75mmol/l
- KCl: 0.075mmol/l
Nominal and measured concentrations:
Due to the limited water solubility of the test item, a supersaturated dispersion of the test item with a loading rate of 100mg/l was continuously stirred at room temperature in the dark over 48 hours. This stock dispersion was filtered and the undiluted filtrate with the maximum concentration of dissolved test item was used as the highest test medium concentration and as the stock solution for dilutions in a geometric series differing by a constant factor of 2. The analytically determined test item concentration in the analyzed test medium samples of the undiluted filtrate amounted to 0.06µg/l at the start of the test and 0.09µg/l at the end of the test. The mean measured test item concentration (calculated as the average over all measurements) was 0.07µg/l.
Reference substance (positive control):
yes
Remarks:
Potassium chromate is testsed as positive control at least once a year to demonstrate satisfactory test conditions.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 0.07 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mobility
Remarks on result:
other: The 48-hour NOEC might even be higher, but concentrations in excess of the loading rate of 100mg/l have not been tested due to the limited water solubility of the test item in the used test water.
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 0.07 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mobility
Remarks on result:
other: The 48-hour EC0 might even be higher, but concentrations in excess of the loading rate of 100mg/l have not been tested due to the limited water solubility of the test item in the used test water.
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.07 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mobility
Remarks on result:
other: EC50 > water solubility

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
no acute toxic efects on Daphnia magna up to the solubility limit