3,4-dichlorophenyl isocyanate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with national standard method with acceptable restrictions

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Version / remarks:

1986

Principles of method if other than guideline:

Method is comparable to OECD TG 209

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge of a predominantly domestic sewage

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Nominal and measured concentrations:

1000, 1800, 3200, 5600 and 10000 mg/l

Details on test conditions:

Type: aquatic

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

1 546 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

3 213 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Validity criteria fulfilled:

not specified

Executive summary:

For 3,4 -dichlorophenyl isocyanate a test with activated sludge with a duration of 30 minutes was performed using a method comparable to OECD TG 209. An EC50 value of 3213 mg/l (nominal) was observed (Bayer, 1988).

Reference 2

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Original reference not available; effect concentration (toxicity threshold) not further specified

Qualifier:

according to guideline

Guideline:

ETAD Fermentation Tube Method

GLP compliance:

no

Specific details on test material used for the study:

3,4-dichlorophenyl isocyanate hydrolyzes rapidly to 3,4-dichloroaniline

Analytical monitoring:

no

Test organisms (species):

other: effluent bacteria

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

24 h

Duration:

24 h

Dose descriptor:

other: SG

Effect conc.:

ca. 600 mg/L

Details on results:

SG: Schädlichkeitsgrenze (toxicity treshold)

Criteria for toxicity unknown.

Validity criteria fulfilled:

not specified

Executive summary:

Hoechst AG, 1982

For freshwater effluent bacteria, a toxicity threshhold of approx. 600 mg/L was observed for an exposure period of 24h. The criteria for toxicity are unknown. The conditions of hydrolysis of 3,4-dichlorophenyl isocyanate towards 1-amino-3,4-dichlorobenzene were described as: 10 g/l, 24 h, pH = 12, 25 °C.

Description of key information

For transported isolated intermediates according to REACh, Article 18, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".

For 3,4-dichlorophenyl isocyanate a test with activated sludge with a duration of 30 minutes was performed using a method comparable to OECD TG 209. An EC50 value of 3213 mg/l (nominal) was observed (Bayer, 1988).

 

For freshwater effluent bacteria, a toxicity threshhold of approx. 600 mg/L was observed for an exposure period of 24h. The criteria for toxicity are unknown. The conditions of hydrolysis of 3,4-Dichlorophenyl isocyanate towards 1-amino-3,4-dichlorobenzene were described as: 10 g/l, 24 h, pH = 12, 25 °C (Hoechst AG, 1982).