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EC number: 221-070-0
CAS number: 2997-92-4
nominal concentration (mg/L)
Immobility 24 hours
Immobility 48 hours
O2-Concentration in mg/L
24 h old
24 h new
measured concentration (mg/L) 0 h
measured concentration (mg/L) 24 h old
measured concentration (mg/L) 24 h new
measured concentration (mg/L) 48 h old
geometric mean (mg/L)
3. measured concentrations
All validity criteria were met. For the
estimation of the EC50s of the test item, the fits showed sufficient
statistical correspondence of the data with the dose-response-equation.
Potassium dichromate K2Cr2O7
(CAS No. 7778 -50 -9) was used as positive control in a current
reference study (201207R201). The value was determined as 1.5 mg/L,
lying within the demanded range of 0.6 -1.7 mg/L. No significant
immobilisation occured in the control.
The recovery after 24 hours ranged from
85 % to 89 % of the start concentration. After 48 hours, recovery ranged
from 81 % to 100 %. The correlation between nominal and measured
concentration was good with exception the lowest concentrated treatment
containing 1.0 mg/L (lower than LOQ). Therefore, the determination of
the biological results was based on the geometric mean of the measured
No observations were made which might
cause doubts concerning the validity of the study outcome. The resukt of
the test is considered valid.
Key value is observed in a semi-static study exposing Daphnia magna for 48 hours to 2, 2'-azobis(2-methylpropionamidine) dihydrochloride. The test solution was changed after 24 hours to maintain the concentration above 80 % of nominal concentration.
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