Registration Dossier
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EC number: 221-070-0 | CAS number: 2997-92-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water and sediment: simulation tests
- Type of information:
- experimental study planned
- Study period:
- as soon as possible after decision from ECHA
- Justification for type of information:
- ESTING PROPOSAL ON VERTEBRATE ANIMALS
[Please provide information for all of the points below. The information should be specific to the endpoint for which testing is proposed. Note that for testing proposals addressing testing on vertebrate animals under the REACH Regulation this document will be published on the ECHA website along with the third party consultation on the testing proposal(s).]
NON-CONFIDENTIAL NAME OF SUBSTANCE:
- Name of the substance on which testing is proposed to be carried out
2,2’-azobis[2-methylpropionamidine] dihydrochloride
- Name of the substance for which the testing proposal will be used [if different from tested substance]
-/-
CONSIDERATIONS THAT THE GENERAL ADAPTATION POSSIBILITIES OF ANNEX XI OF THE REACH REGULATION ARE NOT ADEQUATE TO GENERATE THE NECESSARY INFORMATION [please address all points below]:
- Available GLP studies
No GLP studies regarding biodegradation in surface water of the substance itself or transformation products available
- Available non-GLP studies
No non-GLP studies regarding biodegradation in surface water of the substance itself or transformation products available
- Historical human data
Not relevant
- (Q)SAR
No (Q)SAR for the endpoint simulation of biodegradation in surface water available.
- In vitro methods
Not relevant
- Weight of evidence
No relevant information regarding biodegradation in surface water available.
- Grouping and read-across
No relevant information regarding biodegradation in surface water from similar substances or transformation products available.
- Substance-tailored exposure driven testing [if applicable]
-/-
- Approaches in addition to above [if applicable]
-/-
- Other reasons [if applicable]
-/-
CONSIDERATIONS THAT THE SPECIFIC ADAPTATION POSSIBILITIES OF ANNEXES VI TO X (AND COLUMN 2 THEREOF) OF THE REACH REGULATION ARE NOT ADEQUATE TO GENERATE THE NECESSARY INFORMATION:
The substance itself or its transformation products identified based in the findings in the publication of Werber can be considered as soluble in water. Additionally, findings in the screening tests for biodegradation showed limited degradation under test conditions only, and therefore neither the substance nor (all) transformation products can be considered as readily biodegradable. These conclusions are supported by estimations on biodegradation using the OECD QSAR Toolbox.
FURTHER INFORMATION ON TESTING PROPOSAL IN ADDITION TO INFORMATION PROVIDED IN THE MATERIALS AND METHODS SECTION:
- Details on study design / methodology proposed [if relevant]
The study should be performed at 12 °C in line with the guidance documents. Based on the findings and estimations regarding the partition coefficient of the substance and its transformation products as well as the identified use pattern a relevant exposure of the sediment and/or soil is considered to be unlikely. Therefore, only simulation testing in surface water is proposed.
Data source
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 309 (Aerobic Mineralisation in Surface Water - Simulation Biodegradation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2'-azobis[2-methylpropionamidine] dihydrochloride
- EC Number:
- 221-070-0
- EC Name:
- 2,2'-azobis[2-methylpropionamidine] dihydrochloride
- Cas Number:
- 2997-92-4
- Molecular formula:
- C8H18N6.2ClH
- IUPAC Name:
- 2-[(E)-2-(1-carbamimidoyl-1-methylethyl)diazen-1-yl]-2-methylpropanimidamide dihydrochloride
- Test material form:
- solid: granular
Constituent 1
Study design
- Details on study design:
- TEST CONDITIONS
- Volume of test solution/treatment:
- Composition of medium:
- Additional substrate:
- Solubilising agent (type and concentration if used):
- Test temperature: 12 °C
- pH:
- pH adjusted: yes/no
- CEC (meq/100 g):
- Aeration of dilution water:
- Suspended solids concentration:
- Continuous darkness: yes/no
- Any indication of the test material adsorbing to the walls of the test apparatus:
- Other:
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Method used to control oxygen conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:
SAMPLING
- Sampling frequency:
- Sampling method used per analysis type:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:
DESCRIPTION OF CONTROL AND/OR BLANK TREATMENT PREPARATION
CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:
STATISTICAL METHODS:
Results and discussion
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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