Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 11 October 1996 to 28 November 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study according to OECD guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
SR 48941
Code: 3207
Chemical name: 4-bromomethyl-biphenyl-2-carbonitrile
CAS number: 114772-54-2
Batch: 6 R 00010
Appearence: Whrite to beige powder
Characteristics:
Solubility: practically insoluble in water (<0.01%)
Stability: stable under normal handling conditions
Melting point: 122.4°C
Organic carbon rate: 61.7%

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
1.10^7 microorganisms/ml
30 ml of inoculum are intriduced in each flask.
Duration of test (contact time):
28 d
Details on study design:
Container: serie of glass flasks containing 3L of media.
Test: 2 referenced flasks FE1 and FE2 (SR48941 + inoculum)
Concentration of organic carbon: FE 1 : 10 mg/l (48.8 mg of SR48941) / FE2: 20 mg/l (98.0 mg of SR48941)
Control test: 2 flasks of FB1 and FB2 (inoculum control)
Inoculum control: 1 flask referenced Fc containing aniline (reference substance) at 20 mg/l in organic carbon (procedure control).
Inhibition control: (toxicity control): 1 flask referenced FI in order to verify an eventual inhibitor effect of the product SR48941, containing aniline at 20 mg/l in organic carbon and product at 20 mg/l of organic carbon (weight of SR48941: 98.5 mg).
Abiotic degradation control: 1 flask referenced FA containing the product at 20 mg/l of organic carbon and a sterilising agent (HgCl2) (weight of Sr48941: 99.5 mg).
System control: 1 abiotic control flask referenced FBA (media + HgCl2)
Temperature: 21 +/- 1°C (air-conditionned room, dark)
Aeration: compressed air purified in CO2 (30 to 50 ml/min)
Test duration: 28 days - acidification (HCL conc.) on 27th day
Reference substance
Reference substance:
aniline
Remarks:
20 mg/l of organic carbon

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
0
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
biodegradation: 89% of theorical CO2

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Under the test conditions and the test period, the product SR 48941 is not readily biodegradable.