2,5-diaminobenzenesulphonic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

data is from publication.

Data source

Reference

Reference Type:

publication

Title:

mutagenicity and toxicity studies of p-phenylenediamine and its derivatives;

Author:

Chung,kt

Year:

1995

Bibliographic source:

toxicol. Lett.

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

mutagenicity and toxicity studies of test substance and its derivatives were evaluated for its mutagenic potential.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2,5-diaminobenzenesulphonic acid

EC Number:

201-832-9

EC Name:

2,5-diaminobenzenesulphonic acid

Cas Number:

88-45-9

Molecular formula:

C6H8N2O3S

IUPAC Name:

2,5-diaminobenzene-1-sulfonic acid

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

RAT, LIVER, S-9, AROCLOR 1254

Test concentrations with justification for top dose:

1-3000 UG/PLATE

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

not specified

Details on test system and experimental conditions:

Method: PREINCUBATION

Evaluation criteria:

The plates were observed for histidine revertent mutant colonies.

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Remarks on result:

other: No mutagenic effect were observed

Applicant's summary and conclusion

Conclusions:

The test substance was evaluated for its mutagenic potential in Salmonella typhimurium TA 98 and TA 100 both in the presence and absence of metabolic activation. The test result was considered to be non-mutagenic both in the presence and absence of metabolic activation.

Executive summary:

The test substance was evaluated for its mutagenic potential in Salmonella typhimurium TA 98 and TA 100 both in the presence and absence of metabolic activation. The test substance was exposed to Salmonella typhimurium at the concentration of 1-3000µg/plate in the presence and absence of metabolic activation. No mutagenic effect were observed. Therefore the test substance was considered to be non-mutagenic both in the presence and absence of metabolic activation. Hence the substance cannot be classified as mutagenic in vitro.