2,2'-dithiodi(benzoic acid)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-dithiodi(benzoic acid)

EC Number:

204-352-8

EC Name:

2,2'-dithiodi(benzoic acid)

Cas Number:

119-80-2

Molecular formula:

C14H10O4S2

IUPAC Name:

2-[(2-carboxyphenyl)disulfanyl]benzoic acid

Test material form:

other: Solid

Details on test material:

The test material had a purity of 75.7%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

-Water samples were taken from the middle of test vessels at 0, 48 and 96h

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Upwey Trout Farm, Weymouth, Dorset, UK

ACCLIMATION
- Acclimation period: 11 days
- Type and amount of food: Promin (proprietary product)
-Pretest fasting: 24hours before dosing
-Medication: None given

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

-observations of mortality and toxicity symptoms made at 24, 48, 72, 96 hours

Test conditions

Hardness:

-measurement method: titration with EDTA using BDH total hardness indicator

Test temperature:

-14-16 degrees C
-measurement method: mecury in glass thermometer

pH:

-measurement method: Corning Model 240 meter

Dissolved oxygen:

-measurement method: Yellow Springs Instruments dissolved oxygen meter Mmodel 51B

Nominal and measured concentrations:

-nominal: 180, 100, 56, 32, 18, 10mg DTBA per litre
-measured: 73, 57, 44, 28, 16, 8.1 mg DTBA per litre

Details on test conditions:

TEST SYSTEM
- Test vessel: glass 360mm x 230mm x 230mm 15l capacity

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Water treatment: Passed through activated carbon coarsely filtered to remove particulate material and dechlorinated with sodium thiosulphate, passed through an ultraviolet steriliser to a second set of filters of 25 and 10 microns

TEST CONDITIONS
-photoperiod: 16 hours light/8 hours dark

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

61 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

66 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

69 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

28 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

behaviour

Any other information on results incl. tables

Sublethal observations / clinical signs:

Results:

Measured Concentration	Cumulative Percentage Mortality				Symptoms of Toxicity Observed
	24h	48h	72h	96h	24h	48h	72h	96h
73	20	60	70	80	C/abcdef	C/cefg	C/abcdefh	C/bcef
57	0	20	20	20	C/cef	C/abcefg	C/abcef	C/bcdef
44	0	0	0	0	A	C/cf	C/cef	C/bcef
28	0	0	0	0	A	A	A	A
16	0	0	0	0	A	A	A	A
8.1	0	0	0	0	A	A	A	A
Control	0	0	0	0	A	A	A	A

Key:

A=No significant effect. 10% or less of test population deead or exhibiting symptoms of toxicity

B=Partial effect. 11 -30% of test population dead or exhibiting symptoms of toxicity

C=Significant effect. 30% or more of test population dead or exhibiting symptoms of toxicity

a-quiescence

b-surfacing

c-sounding

d-loss of balance

e-dark discolouration

f-rapid respiration

g-mottled colouration

h-ceased swimming

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 hour LC50 for fish = 61 mg/l. The 96 hour NOEC = 28mg/l