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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment
Reason / purpose:
reference to same study
Qualifier:
no guideline followed
Principles of method if other than guideline:
The acute toxicity of Catalyst 32899 (o-diphenylphosphinobenzoic acid) to the crustacean zooplankter, Daphnia magna, was determined within the framework of the assessment of hazard associated with its manufacture, transport, use and disposal.

Species: Daphnia magna
Exposure regime: Static: 8 groups of ten and a control group for 48 hours
Exposure concentrations: Catalyst 32899 was loaded into the system at concentrations of 1, 2, 5, 10, 20, 50 100 and 200 mg/L
Examinations: Percentage immobilised
GLP compliance:
no
Specific details on test material used for the study:
Referred to in test report as Catalyst 32899 (o-diphenylphosphinobenzoic acid; o-DPPBA) which is equivalent to 2-(diphenylphosphino)benzoic acid
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: Taken from a culture in STL derived from a strain obtained from I.R.Ch.A., France.
- Age at study initiation (mean and range, SD): Less than 24 h old.
- Weight at study initiation (mean and range, SD): Not applicable.
- Length at study initiation (length definition, mean, range and SD): Not applicable.
- Valve height at study initiation, for shell deposition study (mean and range, SD): Not applicable.
- Peripheral shell growth removed prior to test initiation: Not applicable.
- Method of breeding: No data.
- Feeding during test: No data

ACCLIMATION
- Acclimation period: No data

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
230 +/- 20 mg/L
Test temperature:
20 +/- 1°C
pH:
8.2 +/- 0.2
Dissolved oxygen:
9.2 +/- 0.2 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:crystallising dish
- Type (delete if not applicable): No data
- Material, size, headspace, fill volume: 150 mL glass crystallisation dish, filled volume:- 100 mL water.
- Aeration: No data.
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable.
- Renewal rate of test solution (frequency/flow rate): No renewal.
- No. of organisms per vessel: Ten
- No. of vessels per concentration (replicates): Three
- No. of vessels per control (replicates): Three
- No. of vessels per vehicle control (replicates): Not applicable.
- Biomass loading rate: Not applicable.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Filtered, dechloronated mains water.
- Total organic carbon: No data
- Particulate matter: No data
- Metals: No data
- Pesticides: No data
- Chlorine: No data
- Alkalinity: No data
- Ca/mg ratio: No data
- Conductivity: No data
- Culture medium different from test medium: No data
- Intervals of water quality measurement: No data

OTHER TEST CONDITIONS
- Adjustment of pH: No data
- Photoperiod: No data
- Light intensity: No data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : After 24h and 48h the numbers of immobilised D. magna were counted and recorded. D. magna were considered to be immobile if, when the contents of the dish were briefly stirred they did not swim during a 10 second period of observation.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
35 mg/L
Remarks on result:
other: 95% fiducial limits 24 - 53 mg L-1
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.7 mg/L
Remarks on result:
other: 95% fiducial limits 2.7 - 4.9 mg L-1
Details on results:
The catalyst 32899 was not wholly soluble in water over the range of concentrations used in these experiments. At all concentrations tested it formed a powdery deposit on the surface of the water.

Immobilisation of D. magna exposed to a range of concentrations of Catalyst 32899:

Concentration of Catalyst 32899 (mg L-1)

Number of D. magna

Cumulative total immobilised

24h

48h

 

0 (control)

10

0

0

10

0

0

10

0

0

 

1

10

0

2

10

0

1

10

1

2

 

2

10

0

6

10

0

3

10

0

3

 

5

10

1

6

10

0

5

10

2

5

 

10

10

6

8

10

5

7

10

4

9

 

20

10

4

9

10

3

8

10

5

10

 

50

10

4

10

10

5

10

10

4

10

 

100

10

6

10

10

7

10

10

6

10

 

200

10

9

10

10

10

10

10

8

10

Conclusions:
The acute toxicity to the crustacean zooplankter, Daphnia magna, was determined in a static water test without renewal of the test media. The 24 h and 48 h EC50 values were calculated to be 35 mg/L (95% fiducial limits 24 -53 mg/L ) and 3.7 mg/L (95% fiducial limits 2.7 -4.9 mg/L) respectively.
Executive summary:

Catalyst 32899 (o-diphenylphosphinobenzoic acid), a powdery solid, was not wholly soluble in water over the range of concentrations tested.

The acute toxicity to the crustacean zooplankter, Daphnia magna, was determined in a static water test without renewal of the test media. The 24 h and 48 h EC50 values were calculated to be 35 mg L -1 (95% fiducial limits 24 -53 mg/L) and 3.7 mg/L (95% fiducial limits 2.7 -4.9 mg/L) respectively.

Although this acute invertebrate EC50 value is above the predicted water solubility limit for the substance (0.3-1.4 mg/L), the observed toxicity could be attributed to a combination of the test item and the oxide transformation product (2-(diphenylphosphino)benzoic acid oxide) which is predicted to be more soluble in water (79-764 mg/L according to EPISUITE WSKOWWIN and WATERNT).

Description of key information

Catalyst 32899 (o-diphenylphosphinobenzoic acid), a powdery solid, was not wholly soluble in water over the range of concentrations tested.

The acute toxicity to the crustacean zooplankter,Daphnia magna, was determined in a static water test without renewal of the test media. The 24 h and 48 h EC50values were calculated to be 35 mg L-1(95% fiducial limits 24 -53 mg/L) and 3.7 mg/L (95% fiducial limits 2.7 -4.9 mg/L) respectively.

Although this acute invertebrate EC50 value is above the predicted water solubility limit for the substance (0.3-1.4 mg/L), the observed toxicity could be attributed to a combination of the test item and the oxide transformation product (2-(diphenylphosphino)benzoic acid oxide) which is predicted to be more soluble in water (79-764 mg/L according to EPISUITE WSKOWWIN and WATERNT).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
3.7 mg/L

Additional information

Catalyst 32899 (o-diphenylphosphinobenzoic acid), a powdery solid, was not wholly soluble in water over the range of concentrations tested.

The acute toxicity to the crustacean zooplankter, Daphnia magna, was determined in a static water test without renewal of the test media. The 24 h and 48 h EC50values were calculated to be 35 mg/L (95% fiducial limits 24 -53 mg/L) and 3.7 mg/L (95% fiducial limits 2.7 -4.9 mg/L) respectively.