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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Key study: Test method EEC method C.4, OECD Guideline 301B. GLP study. Test item was determined to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Key study: The ready biodegradability of the test substance was assessed in the CO2 Evolution test (Modified Sturm test, EEC Method C.4 and OECD Guideline 301B). 30 mg/L SS of activated sludge were exposed to 10 mgC/L of test item in mineral salts medium (3L) for 36 days under aerobic conditions at temperature of 20 -24 ºC. The obtained results with control vessels confirmed that the inoculum was viable and that the test was valid. Cumulative CO2 production in replicate cultures on Day 28 was equivalent to 51 and 29% degradation. After 36 days of incubation, the mean level of degradation was 62% (level of variation between the replicate cultures of 3% difference). A biodegradation plateau was not achieved by the end of the test on Day 36, and therefore the test item was determined to be not readily biodegradable.