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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)

Data source

Reference
Reference Type:
publication
Title:
Etude toxicologique et physiopathologie du monobromotrifluoroéthane (CF3Br)
Author:
Paulet G.
Year:
1962
Bibliographic source:
Archives des Maladies Professionnelles, extrait du T.23, No.6, 1962, pp.341-348.

Materials and methods

Principles of method if other than guideline:
Acute inhalation toxicity study performed before the issue of testing guidelines.
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: gas
Details on test material:
No data

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
inhalation: gas
Type of inhalation exposure:
whole body
Vehicle:
oxygen
Details on inhalation exposure:
Animals were exposed in a dynamic plexiglas chamber (in open circuit) at successively higher levels of bromotrifluoromethane but with an approximate oxygen level of 20% (between 15 and 20%) close to that contained in air.
The duration of the exposed was fixed to 2 hours. CO2 exhalation was absorbed on a lime filter (preliminary assays have shown no absorption of bromotrifluoromethane by lime).
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
2 h
Concentrations:
300 000, 400 000, 500 000, 600 000, 800 000 (with 20% O2), 850 000 ppm (with 15 or 20% O2)
No. of animals per sex per dose:
10 animals per group
Control animals:
no
Details on study design:
Animals were exposed in an inhalation chamber for 2 hours. Clinical signs were recorded during the inhalation and the two days after.

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LC50
Effect level:
> 800 000 - < 850 000 ppm
Based on:
test mat.
Exp. duration:
2 h
Mortality:
Deaths occurred at 850 000 ppm (with 15% O2)
Clinical signs:
other: See table below.
Body weight:
No data
Gross pathology:
No data

Any other information on results incl. tables

Number of mice

Concentration (ppm)

Exposure duration

Observations

Mortality

Immediate

2 days post- exposure

10

300 000

2

no clinical signs 

 0

10

400 000

2

 No clinical signs

 0

 0

10

500 000

2

Threshold. After a small first reaction, behaviour slightly modified. Hypoactivity, slight loud breathing. Piloerection

10

600 000

2

 0

 0

10

800 000(a)

2

Hypoactivity, dyspnea, feeling of oppression, Sommolence, small tremors, lameness, unsteadiness and gait disorder.

 0

 0

10

850 000 (b)

2

High feeling of oppression, few animals lying on the side. Tremors.

 0

6

10

850 000 (b)

2

High feeling of oppression, few animals lying on the side. Tremors. 

 0

 8

Applicant's summary and conclusion

Interpretation of results:
practically nontoxic
Remarks:
Migrated information
Conclusions:
Whole body inhalation of 2 hours in mice resulted in no mortality up to 800000 ppm. Most of the mice died at 850000 ppm however it was not possible to maintain a level of oxygen of 20%. It is concluded that the median lethal concentration in mice is between 800000 and 850000 ppm.
Executive summary:

10 mice were exposed up to 850 000 ppm (5,270,000 mg/m3) of bromotrifluoromethane in atmosphere for 2 hours. The acute inhalation toxicity of bromotrifluoromethane was very low with no mortality up to 800 000 ppm. Minor clinical signs were observed in the animals exposed up to 600 000 ppm (3,720,000 mg/m3). Mortality with precedent clinical signs (high feeling of oppression, tremors and standing on the side) was observed at 850 000 ppm but it was not possible to maintain a level of 20% oxygen at this concentration. Thus, It is concluded that the median lethal concentration in mice is between 800000 and 850000 ppm.