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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
other: hydrolysed substance
Adequacy of study:
key study
Study period:
1992
Reliability:
1 (reliable without restriction)
Justification for type of information:
Substance 530-62-1 hydrolyses

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: 99%
Specific details on test material used for the study:
99,9%

Method

Target gene:
Method: Standard plate (accord. to Ames et al.) and preincubation
test (accord. to Matsushima et al.)
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Details on test system and experimental conditions:
1st experiment: standard plate test
0, 20, 100, 500, 2500, 5000 µg/plate tested with all tester strains. Solvent was aqua dest. 3 plates per dose and
control.
2nd experiment: preincubation test
0, 100, 500,2500, 5000, 7500 µg/plate tested with TA 100. 3 plates per dose and control.
Positive controls:
2.5 and 10 µg 2-aminoanthracene (+S9, all strains); -S9: 5 µg MNNG (TA 100, TA 1535), 10 µg 4-nitrophenylenediamine (TA 98), 100 µg 9-aminoacridine chloride monohydrate (TA 1537). DMSO was used as solvent.

Results and discussion

Test results
Key result
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified

Any other information on results incl. tables

Result:         Complete solubility of TS in aqua dest. was given.

No bacteriotoxic effect (reduced his- background growth) was noted.

No increase of revertants was seen in the standard plate or preincubation test, with or without S9 mix, in any tester strain.

The mean revertant increase by positive control substances was given.

Applicant's summary and conclusion

Conclusions:
Negative
Complete solubility of TS in aqua dest. was given.
No bacteriotoxic effect (reduced his- background growth) was noted.
No increase of revertants was seen in the standard plate or preincubation test, with or without S9 mix, in any tester strain.
The mean revertant increase by positive control substances was given.