Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
other: hydrolysis product
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
review article or handbook
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
99,76%

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Total exposure duration:
30 min

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Details on results:
Endpoint: growth rate
Exposure period: 72 hour(s)
Unit: mg/l Analytical monitoring: no
NOEC: = 25
LOEC: = 50
EC10: = 63.7
EC50: = 133

Any other information on results incl. tables

- The effects are related to the nominal concentrations effect values based on biomass:

EbC10 (72 h) = 58.7 mg/L

EbC10 (96 h) = 33.6 mg/L

EbC50 (72 h) = 126.8 mg/L

EbC50 (96 h) = 81.8 mg/L

NOEC (72 h) = 10.0 mg/L

LOEC (72 h) = 25.0 mg/L

effect values based on growth rate:

EuC10 (96 h) = 20.0 mg/L

EuC50 (96 h) = 129.6 mg/L

- pH values at test start (w/o algae) and after 96 h (inoculated assays):

concentration (mg/L) pH (0 h) pH (96 h)

control         7.84     7.92

5             7.87     7.97

10             7.86     7.98

25             7.87     7.99

50             7.91     8.01

100             8.00     8.05

250             8.17     8.24

Applicant's summary and conclusion