E17-194T, (P,P-diethyl, monoester with 1,2 propanediol)_

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

17-194T, Lot #: 300567-78-7
Composition: P,P-diethyl, monoester with 1,2 propanediol - 92.9%, CAS #2230512-72-6
Physical Description: Clear, transparent liquid
pH: Neutral to slightly acidic
Stability: Test substance was expected to be stable for the duration of testing.
Expiration Date: aug 30, 2022

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
- Sampling method: When measuring the samples from this study, validity of calibration was controlled by measuring a QC sample (c = 4 mg/L).
- Sample storage conditions before analysis: no

Vehicle:

no

Details on test solutions:

Good quality drinking water is used.
Table of analytical results of water is attached in illustration section below

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Danio rerio
- Strain: HAMILTON-BUCHANAN
- Source: The original animals are obtained in 2019 from the University Heidelberg and used for breeding and production of eggs.
- Age at study initiation (mean and range, SD): sexually immature young fish
- Length at study initiation (length definition, mean, range and SD): 1 – 2 cm
- Weight at study initiation (mean and range, SD):114.3 - 253.9 mg

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test): tap water, 2/12 hours, using neon tubes, Temp 23 °C
- Type and amount of food during acclimation: warm-water fish food
- Feeding frequency during acclimation: two times each working day;
- Health during acclimation (any mortality observed): not mention


FEEDING DURING TEST (as applicable) : 24 hours before the start of the test, the test fish were no longer fed.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

1.18 mmol/L

Test temperature:

23 ± 2 °C

pH:

8.12

Dissolved oxygen:

7.5 mg/L

Conductivity:

270 us/cm

Nominal and measured concentrations:

Nominal 100 mg/L
Measured 94.48 and 94.55 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:aquaria
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass aquaria, maximal volume 12 L, fill volume 7 L
- Aeration: accomplished with glass tubes
- Type of flow-through (e.g. peristaltic or proportional diluter): No, static
- Renewal rate of test solution (frequency/flow rate): No
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): No control
- No. of vessels per vehicle control (replicates): No vehicle used
- Biomass loading rate: 1 vessel, each containing 7 L test solution and 7 fish

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water, supplied by the provider Verbandsgemeindewerke Maikammer.
- Total organic carbon: <0.5 mg/L
- Pesticides: < limit of detection



OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 12/12 hours using neon tubes
- Light intensity: 400 – 800 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : death of the fish

TEST CONCENTRATIONS
- Spacing factor for test concentrations: no, limit test was performed
- Range finding study :In a non GLP pre-test no toxicity was observed against al-gae. In the SDS the EC50 against daphnia was stated as > 100 mg/L. Because of the low toxicity of the test item, this study was performed as a limit test at the concentra-tion 100 mg/L because in most cases the fish is the least sensitive test organism.
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

One valid experiment was performed. The toxicity against Danio rerio was tested using a static test design.
The study was performed as a limit test at the concentration 100 mg/L (nominal concentra-tion). In the treatment and in the control, no fish died or showed abnormal behavior.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using LC-MS -determination. The measured concentrations were 95 % of the nominal concentration at the beginning and 100 % at the end of the test.
LC50> 100 mg/L

Executive summary:

LC50> 100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L