Acetic acid, 2-oxo-, reaction products with ethylenediamine, iron chloride (FeCl3) and phenol, potassium salts

Short-term toxicity to fish

Description of key information

The acute toxicity of of the structurally related UVCB EDDHMA-Fe to fish was investigated in carp and rainbow  trout. The LC50 for a 96 hour exposure was greater than 1000 mg/L for both species, however from the study with rainbow trout 100% mortality was observed at 10000 mg/L.

Additional information

- Key study by Bogers with EDDHMA-Fe, 1992 with carp:

Over a period of 96 hours, the actual concentrations remained constant. Under these conditions, EDDHMA-FeNa did not induce any significant acute effects in carp at the exposure concentration of 1000 mg/L. Hence, the 96h-LC50 for carp exposed to EDDHMA-FeNa was greater than 1000 mg/L, the maximum concentration tested.

- Additional study by Thun wirh EDDHMA-Fe, 1990 with rainbow trout:

In the preliminary test, a mortality of 100 % was observed for a concentration of 10 000 mg/l. At 1000 mg/l of EDDHMA Fe K no mortalities were noted. At concentration levels of 100 mg/l and 1000 mg/l, no mortalities were observed. Because of the deep red colour of the test article solutions, an observation of the fish in these test aquaria was not possible. The LC50 is concluded > 1000 mg/L based on measured concentrations, however due to observations on movement of fish being restricted to the lower part of the test vessels and colouration of fins at > 10 mg/L, a NOEC was concluded at 1 mg/L.

- Supporting study by Basf 2010 with EDDHA-Fe in zebrafish:

The test substance EDDHA-FeNa was tested at 120 mg/L, which was analytically confirmed. The 96 hour LC50 value for EDDHA-FeNa with the zebrafish (Danio rerio) was greater than 120 mg/L based on the nominal concentration of the test substance and greater than 122 mg/L based on the mean of analytically detemined concentrations. The 96 hour NOEC was determined to be greater than or equal to 120 mg/L.

- The supporting acute toxicity study by Ciba-Geigy (1978) with EDDHA-Fe to rainbow trout, carp, catfish and bluegill was performed according to the method reported by Bathe et al. (1974). Various concentrations of EDDHA-FeNa were prepared by adding the test material dissolved in aqua dest. (stock solution), at the appropriate amount ensuring that the total volume in each vessel remained the same. The LC50 for a 96 hour exposure was greater than 100 mg/L for Rainbow trout, Carp, Catfish and Bluegill.

- Additionally, a study investigating the acute toxicity of EDDHMA-Na4 to bluegill is given (Engstrom, 1986) as supporting evidence that these similar chelate structures have no high acute toxicity to fish. In this study the LC50 (96h) proved > 1420 mg/L the highest concentration tested, however some mortality was observed at this concentration.