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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- solid: flakes
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Mitsubishi-Chemical Food Corporation; 63281211
- Expiration date of the lot/batch: March 27, 2018
- Purity test date: May 30, 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: below 15 degrees C in a cool, dark, well-ventilated space with container tightly closed
- Solubility and stability of the test substance in the solvent/vehicle: forms viscous solution or gel with water
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sample storage conditions before analysis: -25 degrees C
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test solutions were prepared by adding 100.5 mg of test substance to 1 L dilution water. After 24 hrs, 100.3 mg test substance was added to an additional 1 L of dilution water. This was ultrasonicated fro 2 hrs, and stirred for 72 hrs at room temperature. It was then filtered through a 0.22 um PES bottle top filter. This stock concentration was used as the hightest test concentration, and was diluted for the further tests.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Justification for species other than prescribed by test guideline:
- Source: German Federal Environmental Agency, Institut fur Wasser-, Boden- und Lufthygiene
- Age of parental stock (mean and range, SD): 3 weeks
- Feeding during test: No
ACCLIMATION
- Acclimation period: 1 week
- Acclimation conditions (same as test or not): yes
- Type and amount of food: algal suspension and ArtemioFluid
- Feeding frequency: daily
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Separated by sieving and transported with a bore Pasteur pipette.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 18-22 degrees C
- pH:
- 7.13-8.19
- Dissolved oxygen:
- 7.3-9.2 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 6.25, 12.5, 25.0, 50.0, and 100 mg/L (due to difficulties analyzing the test substance, only nominal concentrations were reported)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Type (delete if not applicable): closed by covering with glass panes
- Material, size, headspace, fill volume: glass, 50 mL fill volume
- Aeration: No, glass panes over the top of the vessel
- Renewal rate of test solution (frequency/flow rate): one renewal at 24 hrs
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Cu-reduced dilution water
OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark
- Light intensity: 807-813 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobility daily
RANGE-FINDING STUDY
- Test concentrations: First study - 1.0, 10.0, and 100 mg/L; second study - 0.1, 1.0, and 10 mg/L
- Results used to determine the conditions for the definitive study: In the first range-finding study, 45% of the daphnids in the 1.0 mg/L concentration died, but only 10% of daphnids at the higher concentrations died. In the second range-finding study, no daphnids died, thus it was decided that in the first range-finding study either the test solutions were improperly made, or the daphnids were not in good condition at the start of the test. The decision was then made to test at the higher concentrations in the first test.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: only one daphnid in the 100 mg/L test concentration was immobilized
- Other biological observations: no significant signs of disease or stress were noted
- Mortality of control: 0
Any other information on results incl. tables
Immobilization of Daphnids after 48 hrs Exposure
Concentration (mg/L) |
Total Daphnids |
Mobile |
Immobile |
% Immobility |
0 |
20 |
20 |
0 |
0 |
6.25 |
20 |
20 |
0 |
0 |
12.5 |
20 |
20 |
0 |
0 |
25.0 |
20 |
20 |
0 |
0 |
50.0 |
20 |
20 |
0 |
0 |
100.0 |
20 |
19 |
1 |
5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hr LOEC for Daphnia magna was > 100 mg/L.
- Executive summary:
The toxicity of the test substance to aquatic invertebrates was determined in an OECD Guideline 202 study. 20 daphnids (5 animals x 4 replicates) were exposed to concentrations of 0, 6.25, 12.5, 25.0, 50.0, and 100 mg/L for 48 hrs. Only one daphnid in the 100 mg/L group died. Due to difficulties with analysis of the test substance, only nominal concentrations were reported. The LOEC was > 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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